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Plasmid Files

pIDK

Baculovirus transfer vector with a p10 promoter and a kanamycin resistance marker. Serves as a donor vector in the MultiBac™ system.

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pIDK Sequence and MappIDK.dna
Map and Sequence File   
Sequence Author:  Geneva Biotech
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 BspDI - ClaI (2280) AvrII (2272) PI-SceI (2247) PfoI (2165) BstBI (2072) EciI (1919) RsrII (1906) MslI (1827) BssHII (1787) BpuEI (1766) BsrDI (1623) NmeAIII (1614) BciVI (1609) PflFI - Tth111I (1508) FspI (1492) MscI (1472) PstI (1443) SfcI (1439) DrdI (1417) BsiEI (1299) EagI (1296) BglII (1226) XcmI (1206) SpeI (4) AccI (9) BstZ17I (10) PacI (86) BbsI (139) PaeR7I - XhoI (153) BtgI - NcoI (159) NheI (166) BmtI (170) NsiI (181) Acc65I (190) KpnI (194) BsaI (352) PmeI (490) BstXI (502) SnaBI (548) PsiI (757) HindIII (788) AflIII (960) AlwNI (1014) Bts α I (1075) pIDK 2281 bp
BspDI  (2280)
1 site
A T C G A T T A G C T A
ClaI  (2280)
1 site
A T C G A T T A G C T A
AvrII  (2272)
1 site
C C T A G G G G A T C C
PI-SceI  (2247)
1 site
A T C T A T G T C G G G T G C G G A G A A A G A G G T A A T G A A A T G G T A G A T A C A G C C C A C G C C T C T T T C T C C A T T A C T T T A C C

PI-SceI  is a homing endonuclease that can recognize a variety of
similar recognition sequences.
PfoI  (2165)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (2072)
1 site
T T C G A A A A G C T T
EciI  (1919)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
RsrII  (1906)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MslI  (1827)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BssHII  (1787)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BpuEI  (1766)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrDI  (1623)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
NmeAIII  (1614)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BciVI  (1609)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
PflFI  (1508)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1508)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (1492)
1 site
T G C G C A A C G C G T
MscI  (1472)
1 site
T G G C C A A C C G G T
PstI  (1443)
1 site
C T G C A G G A C G T C
SfcI  (1439)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
DrdI  (1417)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BsiEI  (1299)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EagI  (1296)
1 site
C G G C C G G C C G G C
BglII  (1226)
1 site
A G A T C T T C T A G A
XcmI  (1206)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SpeI  (4)
1 site
A C T A G T T G A T C A
AccI  (9)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BstZ17I  (10)
1 site
G T A T A C C A T A T G
PacI  (86)
1 site
T T A A T T A A A A T T A A T T
BbsI  (139)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PaeR7I  (153)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (153)
1 site
C T C G A G G A G C T C
BtgI  (159)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (159)
1 site
C C A T G G G G T A C C
NheI  (166)
1 site
G C T A G C C G A T C G
BmtI  (170)
1 site
G C T A G C C G A T C G
NsiI  (181)
1 site
A T G C A T T A C G T A
Acc65I  (190)
1 site
G G T A C C C C A T G G
KpnI  (194)
1 site
G G T A C C C C A T G G
BsaI  (352)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PmeI  (490)
1 site
G T T T A A A C C A A A T T T G
BstXI  (502)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SnaBI  (548)
1 site
T A C G T A A T G C A T
PsiI  (757)
1 site
T T A T A A A A T A T T
HindIII  (788)
1 site
A A G C T T T T C G A A
AflIII  (960)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
AlwNI  (1014)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BtsαI  (1075)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
NeoR/KanR
1262 .. 2056  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
1262 .. 2056  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
R6K γ ori
545 .. 901  =  357 bp
γ replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication
R6K γ ori
545 .. 901  =  357 bp
γ replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication
p10 promoter
15 .. 124  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
15 .. 124  =  110 bp
baculovirus promoter for expression in insect cells
MCS
132 .. 195  =  64 bp
multiple cloning site
MCS
132 .. 195  =  64 bp
multiple cloning site
HSV TK poly(A) signal
260 .. 308  =  49 bp
herpes simplex virus thymidine kinase
polyadenylation signal
HSV TK poly(A) signal
260 .. 308  =  49 bp
herpes simplex virus thymidine kinase
polyadenylation signal
loxP
510 .. 543  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
510 .. 543  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
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