pIEx-2

Vector for high-level expression in insect cells of proteins with a cleavable N-terminal GST-6xHis-S-Tag cassette plus a C-terminal HSV tag.

Sequence Author: MilliporeSigma (Novagen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
DrdI (4095) PspFI (3897) BseYI (3893) AhdI (3309) BsaI (3243) BpmI (3240) NmeAIII (3162) AatII (2390) ZraI (2388) XbaI (2382) PflFI - Tth111I (2327) BspDI * - ClaI * (207) EcoRV (239) PsiI (409) NheI (606) BmtI (610) SphI (614) BsmI (775) BsiWI (784) MluI (872) BtgI - NcoI (1082) EcoNI (1097) EcoO109I (1119) MscI (1294) BsgI (1373) SwaI (1514) SpeI (1753) 6xHis XcmI (1786) PflMI (1830) thrombin site PshAI (1904) BseRI (1908) BamHI (1926) MfeI (1932) Eco53kI (1941) BanII - SacI (1943) BsrGI (1946) AscI (1954) PstI - SbfI (1964) SalI (1966) Acc65I (1972) AgeI (1975) KpnI (1976) HindIII (1984) NotI (1991) BstZ17I (2006) PmlI (2011) BbsI (2045) PaeR7I - XhoI (2053) DraIII (2064) Bsu36I (2074) PacI (2103) IE1 terminator pIEx™-2 4563 bp
DrdI  (4095)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PspFI  (3897)
1 site
C C C A G C G G G T C G
BseYI  (3893)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AhdI  (3309)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (3243)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BpmI  (3240)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
NmeAIII  (3162)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AatII  (2390)
1 site
G A C G T C C T G C A G
ZraI  (2388)
1 site
G A C G T C C T G C A G
XbaI  (2382)
1 site
T C T A G A A G A T C T
PflFI  (2327)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2327)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BspDI  (207)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (207)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
EcoRV  (239)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PsiI  (409)
1 site
T T A T A A A A T A T T
NheI  (606)
1 site
G C T A G C C G A T C G
BmtI  (610)
1 site
G C T A G C C G A T C G
SphI  (614)
1 site
G C A T G C C G T A C G
BsmI  (775)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BsiWI  (784)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
MluI  (872)
1 site
A C G C G T T G C G C A
BtgI  (1082)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (1082)
1 site
C C A T G G G G T A C C
EcoNI  (1097)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
EcoO109I  (1119)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
MscI  (1294)
1 site
T G G C C A A C C G G T
BsgI  (1373)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SwaI  (1514)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
SpeI  (1753)
1 site
A C T A G T T G A T C A
XcmI  (1786)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PflMI  (1830)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
PshAI  (1904)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BseRI  (1908)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BamHI  (1926)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
MfeI  (1932)
1 site
C A A T T G G T T A A C
Eco53kI  (1941)
1 site
G A G C T C C T C G A G
BanII  (1943)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (1943)
1 site
G A G C T C C T C G A G
BsrGI  (1946)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AscI  (1954)
1 site
G G C G C G C C C C G C G C G G
PstI  (1964)
1 site
C T G C A G G A C G T C
SbfI  (1964)
1 site
C C T G C A G G G G A C G T C C
SalI  (1966)
1 site
G T C G A C C A G C T G
Acc65I  (1972)
1 site
G G T A C C C C A T G G
AgeI  (1975)
1 site
A C C G G T T G G C C A
KpnI  (1976)
1 site
G G T A C C C C A T G G
HindIII  (1984)
1 site
A A G C T T T T C G A A
NotI  (1991)
1 site
G C G G C C G C C G C C G G C G
BstZ17I  (2006)
1 site
G T A T A C C A T A T G
PmlI  (2011)
1 site
C A C G T G G T G C A C
BbsI  (2045)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PaeR7I  (2053)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2053)
1 site
C T C G A G G A G C T C
DraIII  (2064)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
Bsu36I  (2074)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PacI  (2103)
1 site
T T A A T T A A A A T T A A T T
GST
1084 .. 1740  =  657 bp
219 amino acids  =  25.6 kDa
Product: glutathione S-transferase from Schistosoma japonicum
GST
1084 .. 1740  =  657 bp
219 amino acids  =  25.6 kDa
Product: glutathione S-transferase from Schistosoma japonicum
6xHis
1768 .. 1785  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
1768 .. 1785  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
S-Tag
1795 .. 1839  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from pancreatic ribonuclease A
S-Tag
1795 .. 1839  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from pancreatic ribonuclease A
thrombin site
1855 .. 1872  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
thrombin site
1855 .. 1872  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
enterokinase site
1891 .. 1905  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
1891 .. 1905  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
HSV tag
2020 .. 2052  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
HSV tag
2020 .. 2052  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
AmpR
2522 .. 3382  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2522 .. 2590  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2522 .. 3382  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2591 .. 3382  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2522 .. 3382  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
IE1 promoter
487 .. 1078  =  592 bp
promoter of the ie1 gene from the baculovirus Autographa californica
IE1 promoter
487 .. 1078  =  592 bp
promoter of the ie1 gene from the baculovirus Autographa californica
ori
3553 .. 4141  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3553 .. 4141  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hr5 enhancer
1 .. 483  =  483 bp
baculovirus early transcription enhancer
hr5 enhancer
1 .. 483  =  483 bp
baculovirus early transcription enhancer
IE1 terminator
2080 .. 2387  =  308 bp
terminator of the ie1 gene from the baculovirus Autographa californica
IE1 terminator
2080 .. 2387  =  308 bp
terminator of the ie1 gene from the baculovirus Autographa californica
AmpR promoter
2417 .. 2521  =  105 bp
AmpR promoter
2417 .. 2521  =  105 bp
MCS
1900 .. 2014  =  115 bp
multiple cloning site
MCS
1900 .. 2014  =  115 bp
multiple cloning site
ORF:  1084 .. 2064  =  981 bp
ORF:  326 amino acids  =  36.9 kDa
ORF:  1745 .. 2068  =  324 bp
ORF:  107 amino acids  =  12.0 kDa
ORF:  2522 .. 3382  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2986 .. 3252  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  452 .. 1090  =  639 bp
ORF:  212 amino acids  =  24.2 kDa
Click here to try SnapGene

Download pIEx-2.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.