Resources
Plasmid Files

pIEx™-8 Ek/LIC (linearized)

Linearized insect cell vector for ligation-independent cloning (LIC) to express proteins with an N-terminal Strep-Tag® II-enterokinase cassette and a C-terminal 10xHis tag.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pIEx-8 Ek_LIC (linearized) Sequence and MappIEx-8 Ek_LIC (linearized).dna
Map and Sequence File   
Sequence Author:  Novagen (EMD Millipore)
Download Free Trial Get SnapGene Viewer

 BmeT110I (2552) AvaI - BsoBI - TspMI - XmaI (2551) BspQI - SapI (2307) PciI (2190) DrdI (2088) PspFI (1890) BseYI (1886) AlwNI (1781) AhdI (1302) BsaI (1236) BsrFI (1217) NmeAIII (1155) ScaI (821) XmnI (702) SmaI (2553) BspDI * - ClaI * (2763) EcoRV (2795) PsiI (2965) NheI (3162) BmtI (3166) SphI (3170) BsmI (3331) BclI * (3334) BsiWI (3340) EagI (3398) MluI (3428) HincII (3600) BtgI - NcoI (3638) ATG BstBI (3665) End (3681) Start (0) DraIII (57) Bsu36I (67) PacI (96) PflFI - Tth111I (320) BtgZI (343) XbaI (375) ZraI (381) AatII (383) pIEx™-8 Ek/LIC 3681 bp
BmeT110I  (2552)
1 site
C Y C G R G G R G C Y C
AvaI  (2551)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (2551)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
TspMI  (2551)
1 site
C C C G G G G G G C C C
XmaI  (2551)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BspQI  (2307)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2307)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
PciI  (2190)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
DrdI  (2088)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PspFI  (1890)
1 site
C C C A G C G G G T C G
BseYI  (1886)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
AlwNI  (1781)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (1302)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (1236)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsrFI  (1217)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
NmeAIII  (1155)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ScaI  (821)
1 site
A G T A C T T C A T G A
XmnI  (702)
1 site
G A A N N N N T T C C T T N N N N A A G
SmaI  (2553)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BspDI  (2763)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2763)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
EcoRV  (2795)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
PsiI  (2965)
1 site
T T A T A A A A T A T T
NheI  (3162)
1 site
G C T A G C C G A T C G
BmtI  (3166)
1 site
G C T A G C C G A T C G
SphI  (3170)
1 site
G C A T G C C G T A C G
BsmI  (3331)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BclI  (3334)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsiWI  (3340)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
EagI  (3398)
1 site
C G G C C G G C C G G C
MluI  (3428)
1 site
A C G C G T T G C G C A
HincII  (3600)
1 site
G T Y R A C C A R Y T G
BtgI  (3638)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (3638)
1 site
C C A T G G G G T A C C
BstBI  (3665)
1 site
T T C G A A A A G C T T
End  (3681)
0 sites
Start  (0)
0 sites
DraIII  (57)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
Bsu36I  (67)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PacI  (96)
1 site
T T A A T T A A A A T T A A T T
PflFI  (320)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (320)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BtgZI  (343)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
XbaI  (375)
1 site
T C T A G A A G A T C T
ZraI  (381)
1 site
G A C G T C C T G C A G
AatII  (383)
1 site
G A C G T C C T G C A G
AmpR
515 .. 1375  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   515 .. 583  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
515 .. 1375  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   584 .. 1375  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
515 .. 1375  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
IE1 promoter
3043 .. 3634  =  592 bp
promoter of the ie1 gene from the baculovirus
Autographa californica
IE1 promoter
3043 .. 3634  =  592 bp
promoter of the ie1 gene from the baculovirus
Autographa californica
ori
1546 .. 2134  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1546 .. 2134  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
hr5 enhancer
2557 .. 3039  =  483 bp
baculovirus early transcription enhancer
hr5 enhancer
2557 .. 3039  =  483 bp
baculovirus early transcription enhancer
IE1 terminator
73 .. 380  =  308 bp
terminator of the ie1 gene from the baculovirus
Autographa californica
IE1 terminator
73 .. 380  =  308 bp
terminator of the ie1 gene from the baculovirus
Autographa californica
AmpR promoter
410 .. 514  =  105 bp
AmpR promoter
410 .. 514  =  105 bp
10xHis
25 .. 54  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
25 .. 54  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
Strep-Tag II
3649 .. 3672  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an
engineered form of streptavidin
Strep-Tag II
3649 .. 3672  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an
engineered form of streptavidin
enterokinase site
3679 .. 3693  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
3679 .. 3693  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
ATG
3640 .. 3642  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
3640 .. 3642  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter