pTriEx-5 Ek_LIC (linearized)

Linearized insect, bacterial, and mammalian vector for ligation-independent cloning (LIC), encoding an N-terminal Strep-Tag® II-enterokinase and a C-terminal 10xHis.

Sequence Author: MilliporeSigma (Novagen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
Bsu36I (67) NcoI (4961) BstBI (4988) End (5004) Start (0) DraIII (57) pTriEx™-5 Ek/LIC 5004 bp
Bsu36I  (67)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4961)
1 site
C C A T G G G G T A C C
BstBI  (4988)
1 site
T T C G A A A A G C T T
End  (5004)
0 sites
Start  (0)
0 sites
DraIII  (57)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1927 .. 2718  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2719 .. 2787  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
3030 .. 3857  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3030 .. 3857  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
412 .. 1117  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
412 .. 1117  =  706 bp
contains part of ORF1629
ori
1327 .. 1914  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1327 .. 1914  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
Exon 1
4446 .. 4547  =  102 bp
Exon 1
4446 .. 4547  =  102 bp
intron
4548 .. 4711  =  164 bp
intron
4548 .. 4711  =  164 bp
Exon 2
4712 .. 5016  =  305 bp
Exon 2
4712 .. 5016  =  305 bp
CMV enhancer
3925 .. 4228  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3925 .. 4228  =  304 bp
human cytomegalovirus immediate early enhancer
Exon 2
1 .. 233  =  233 bp
Exon 2
1 .. 233  =  233 bp
CMV promoter
4230 .. 4429  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4230 .. 4429  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
T7 terminator
352 .. 399  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
352 .. 399  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
ORF603
3192 .. 3797  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
3192 .. 3797  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
529 .. 1117  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
529 .. 1117  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
lef2
3030 .. 3154  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
3030 .. 3154  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
231 .. 342  =  112 bp
rabbit globin terminator
231 .. 342  =  112 bp
p10 promoter
4839 .. 4948  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
4839 .. 4948  =  110 bp
baculovirus promoter for expression in insect cells
ATG
4963 .. 4965  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
4963 .. 4965  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Strep-Tag II
4972 .. 4995  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
Strep-Tag II
4972 .. 4995  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
enterokinase site
5002 .. 5016  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
5002 .. 5016  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
10xHis
25 .. 54  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
25 .. 54  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
lac operator
4799 .. 4823  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
4799 .. 4823  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
4780 .. 4798  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
4780 .. 4798  =  19 bp
promoter for bacteriophage T7 RNA polymerase
poly(A) signal
209 .. 214  =  6 bp
poly(A) signal
209 .. 214  =  6 bp
RBS
4950 .. 4955  =  6 bp
ribosome binding site
RBS
4950 .. 4955  =  6 bp
ribosome binding site
ORF:  2057 .. 2323  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2837 .. 3154  =  318 bp
ORF:  105 amino acids  =  12.1 kDa
ORF:  529 .. 1146  =  618 bp
ORF:  205 amino acids  =  23.4 kDa
ORF:  1927 .. 2787  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3192 .. 3797  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  3236 .. 3466  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
ORF:  4436 .. 4690  =  255 bp
ORF:  84 amino acids  =  9.0 kDa
Click here to try SnapGene

Download pTriEx-5 Ek_LIC (linearized).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.