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Plasmid Files

pTriEx™-6 3C/LIC (linearized)

Linearized insect, bacterial, and mammalian vector for ligation-independent cloning (LIC), encoding an N-terminal Strep-Tag® II-HRV 3C and a C-terminal 10xHis.

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pTriEx-6 3C_LIC (linearized) Sequence and MappTriEx-6 3C_LIC (linearized).dna
Map and Sequence File   
Sequence Author:  Novagen (EMD Millipore)
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 Bsu36I (71) NcoI (4965) BstBI (4992) End (5020) Start (0) DraIII (61) pTriEx™-6 3C/LIC 5020 bp
Bsu36I  (71)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4965)
1 site
C C A T G G G G T A C C
BstBI  (4992)
1 site
T T C G A A A A G C T T
End  (5020)
0 sites
Start  (0)
0 sites
DraIII  (61)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AmpR
1931 .. 2791  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1931 .. 2722  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1931 .. 2791  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   2723 .. 2791  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1931 .. 2791  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
baculovirus recombination region (lef2/ORF603)
3034 .. 3861  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3034 .. 3861  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
416 .. 1121  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
416 .. 1121  =  706 bp
contains part of ORF1629
ori
1331 .. 1918  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1331 .. 1918  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
Exon 2
4716 .. 5031  =  316 bp
Exon 2
4716 .. 5031  =  316 bp
CMV enhancer
3929 .. 4232  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3929 .. 4232  =  304 bp
human cytomegalovirus immediate early enhancer
Exon 2
1 .. 237  =  237 bp
Exon 2
1 .. 237  =  237 bp
CMV promoter
4234 .. 4433  =  200 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
4234 .. 4433  =  200 bp
human cytomegalovirus (CMV) immediate early
promoter
intron
4552 .. 4715  =  164 bp
intron
4552 .. 4715  =  164 bp
Exon 1
4450 .. 4551  =  102 bp
Exon 1
4450 .. 4551  =  102 bp
T7 terminator
356 .. 403  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
356 .. 403  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
ORF603
3196 .. 3801  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
3196 .. 3801  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
533 .. 1121  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
533 .. 1121  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
lef2
3034 .. 3158  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
3034 .. 3158  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
235 .. 346  =  112 bp
rabbit globin terminator
235 .. 346  =  112 bp
p10 promoter
4843 .. 4952  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
4843 .. 4952  =  110 bp
baculovirus promoter for expression in insect cells
10xHis
29 .. 58  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
29 .. 58  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
lac operator
4803 .. 4827  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
4803 .. 4827  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
Strep-Tag II
4976 .. 4999  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an
engineered form of streptavidin
Strep-Tag II
4976 .. 4999  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an
engineered form of streptavidin
HRV 3C site
5006 .. 5029  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human
rhinovirus 3C and PreScission proteases
HRV 3C site
5006 .. 5029  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human
rhinovirus 3C and PreScission proteases
T7 promoter
4784 .. 4802  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
4784 .. 4802  =  19 bp
promoter for bacteriophage T7 RNA polymerase
thrombin site
5 .. 22  =  18 bp
6 amino acids  =  627.8 Da
Product: thrombin recognition and cleavage site
thrombin site
5 .. 22  =  18 bp
6 amino acids  =  627.8 Da
Product: thrombin recognition and cleavage site
poly(A) signal
213 .. 218  =  6 bp
poly(A) signal
213 .. 218  =  6 bp
RBS
4954 .. 4959  =  6 bp
ribosome binding site
RBS
4954 .. 4959  =  6 bp
ribosome binding site
ATG
4967 .. 4969  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
4967 .. 4969  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
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