Resources
Plasmid Files

pVL-FH

Baculovirus transfer plasmid for high-level expression of proteins with a C-terminal FLAG®-8xHis cassette.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pVL-FH.dna
Map and Sequence File:    Download    Open   
Sequence Author:  AB Vector
Download Free Trial Get SnapGene Viewer

´╗┐
NdeI (9509) ScaI (8818) AlwNI (7861) PspFI (7753) BseYI (7749) BspQI - SapI (7329) FspAI (6412) SgrDI (6259) SgrAI (5990) SnaBI (4902) KpnI (4722) PasI (486) PacI (583) XcmI (746) BtgI (868) SacII (871) BstEII (923) PspOMI (1395) ApaI - BanII (1399) PaeR7I - PspXI - XhoI (1901) SphI (2135) BclI * (2232) BbvCI - Bpu10I (3405) NgoMIV (3770) NaeI (3772) EcoRV (4000) BglII (4134) PstI (4142) EagI - NotI (4144) EcoRI (4155) TspMI - XmaI (4160) SmaI (4162) BamHI (4164) XbaI (4172) AvrII (4246) KflI - PpuMI (4271) Acc65I (4718) pVL-FH 9723 bp
NdeI  (9509)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
ScaI  (8818)
1 site
A G T A C T T C A T G A
AlwNI  (7861)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (7753)
1 site
C C C A G C G G G T C G
BseYI  (7749)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (7329)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7329)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (6412)
1 site
R T G C G C A Y Y A C G C G T R
SgrDI  (6259)
1 site
C G T C G A C G G C A G C T G C
SgrAI  (5990)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SnaBI  (4902)
1 site
T A C G T A A T G C A T
KpnI  (4722)
1 site
G G T A C C C C A T G G
PasI  (486)
1 site
C C C W G G G G G G W C