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pBI-CMV5

Mammalian vector for expressing a protein of interest together with secreted Metridia luciferase.

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pBI-CMV5.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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SacI (3717) Eco53kI (3715) EcoRI (3680) AleI (3651) EcoNI (3632) BclI * (3511) XcmI (3476) SmaI (3366) TspMI - XmaI (3364) Bpu10I (3345) BsrGI (3310) AarI - BfuAI - BspMI (3307) PluTI (3249) SfoI (3247) NarI (3246) KasI (3245) ApaI (3015) PspOMI (3011) BglII (3005) Bsu36I (2999) PstI (2996) XbaI (2986) SspI (2676) EarI (2667) XmnI (2471) ScaI (2352) PvuI (2242) FspI (2094) NmeAIII (2020) StuI (3730) PaeR7I - PspXI - XhoI (1) NdeI (240) BsaAI - SnaBI (346) BamHI (602) MluI (620) NheI (626) BmtI (630) EagI - NotI (633) BspDI - ClaI (641) HindIII (646) SalI (652) AccI (653) EcoRV (660) PciI (979) NspI (983) DrdI (1087) BseYI (1283) PspFI (1287) AlwNI (1395) AhdI (1872) pBI-CMV5 3759 bp
SacI  (3717)
1 site
G A G C T C C T C G A G
Eco53kI  (3715)
1 site
G A G C T C C T C G A G
EcoRI  (3680)
1 site
G A A T T C C T T A A G
AleI  (3651)
1 site
C A C N N N N G T G G T G N N N N C A C
EcoNI  (3632)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BclI  (3511)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
XcmI  (3476)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SmaI  (3366)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (3364)
1 site
C C C G G G G G G C C C
XmaI  (3364)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
Bpu10I  (3345)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsrGI  (3310)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AarI  (3307)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (3307)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3307)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PluTI  (3249)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3247)
1 site
G G C G C C C C G C G G
NarI  (3246)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3245)
1 site
G G C G C C C C G C G G
ApaI  (3015)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (3011)
1 site
G G G C C C C C C G G G
BglII  (3005)
1 site
A G A T C T T C T A G A
Bsu36I  (2999)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PstI  (2996)
1 site
C T G C A G G A C G T C
XbaI  (2986)
1 site
T C T A G A A G A T C T
SspI  (2676)
1 site
A A T A T T T T A T A A
EarI  (2667)
1 site
C T C T T C N G A G A A G N N N N