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Plasmid Files

pBIND-ERα

Renilla luciferase vector encoding the Gal4 DNA-binding domain fused to an estrogen receptor ligand-binding domain.

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pBIND-ER(alpha).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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MluI (7481) PciI (6504) AhdI (6334) BspDI - ClaI (5289) BstXI (5229) RsrII (5024) NaeI (5010) NgoMIV (5008) BssHII (4905) PluTI (4511) SfoI (4509) NarI (4508) KasI (4507) EcoO109I (4244) NruI (4060) Bsu36I (3985) EcoRV (3884) SpeI (152) NdeI (387) NheI (1052) BmtI (1056) PaeR7I - XhoI (1300) AsiSI - PvuI (1558) BsmBI (2346) DraI - PmeI (2460) EcoRI (2465) TspMI - XmaI (2481) SmaI (2483) SbfI (2508) NotI (2603) PsiI (2726) MfeI (2755) SexAI * (3100) SfiI (3286) AvrII (3333) DraIII (3587) pBIND-ER α 7605 bp
MluI  (7481)
1 site
A C G C G T T G C G C A
PciI  (6504)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (6334)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspDI  (5289)
1 site
A T C G A T T A G C T A
ClaI  (5289)
1 site
A T C G A T T A G C T A
BstXI  (5229)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
RsrII  (5024)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NaeI  (5010)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (5008)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BssHII  (4905)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PluTI  (4511)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4509)
1 site
G G C G C C C C G C G G
NarI  (4508)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4507)
1 site
G G C G C C C C G C G G
EcoO109I  (4244)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
NruI  (4060)
1 site
T C G C G A A G C G C T
Bsu36I  (3985)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EcoRV  (3884)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
SpeI  (152)
1 site
A C T A G T T G A T C A
NdeI  (387)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NheI  (1052)
1 site
G C T A G C C G A T C G
BmtI  (1056)
1 site
G C T A G C C G A T C G
PaeR7I  (1300)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1300)
1 site
C T C G A G G A G C T C
AsiSI  (1558)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1558)
1 site
C G A T C G G C T A G C
BsmBI  (2346)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
DraI  (2460)
1 site
T T T A A A A A A T T T
PmeI  (2460)
1 site
G T T T A A A C C A A A T T T G
EcoRI  (2465)
1 site
G A A T T C C T T A A G
TspMI  (2481)
1 site
C C C G G G G G G C