pBIND-GR

Renilla luciferase vector encoding the Gal4 DNA-binding domain fused to the glucocorticoid receptor ligand-binding domain.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
BglII (7543) MluI (7424) AhdI (6277) BspDI - ClaI (5232) poly(A) signal BstXI (5172) RsrII (4967) NaeI (4953) NgoMIV (4951) BssHII (4848) PluTI (4454) SfoI (4452) NarI (4451) KasI (4450) EcoO109I (4187) PasI (4116) NruI (4003) EcoRV (3827) SpeI (152) CMV enhancer NdeI (387) T7 promoter NheI (1052) BmtI (1056) PaeR7I - XhoI (1300) GAL4 DNA binding domain AsiSI - PvuI (1558) XcmI (1729) DraI - PmeI (2403) TspMI - XmaI (2424) SmaI (2426) XbaI (2435) SbfI (2451) BlpI (2516) NotI (2546) PsiI (2669) MfeI (2698) SfiI (3229) StuI (3275) AvrII (3276) PflMI (3330) DraIII (3530) pBIND-GR 7548 bp
BglII  (7543)
1 site
A G A T C T T C T A G A
MluI  (7424)
1 site
A C G C G T T G C G C A
AhdI  (6277)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspDI  (5232)
1 site
A T C G A T T A G C T A
ClaI  (5232)
1 site
A T C G A T T A G C T A
BstXI  (5172)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
RsrII  (4967)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NaeI  (4953)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4951)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BssHII  (4848)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PluTI  (4454)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4452)
1 site
G G C G C C C C G C G G
NarI  (4451)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4450)
1 site
G G C G C C C C G C G G
EcoO109I  (4187)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PasI  (4116)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
NruI  (4003)
1 site
T C G C G A A G C G C T
EcoRV  (3827)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
SpeI  (152)
1 site
A C T A G T T G A T C A
NdeI  (387)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NheI  (1052)
1 site
G C T A G C C G A T C G
BmtI  (1056)
1 site
G C T A G C C G A T C G
PaeR7I  (1300)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1300)
1 site
C T C G A G G A G C T C
AsiSI  (1558)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1558)
1 site
C G A T C G G C T A G C
XcmI  (1729)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
DraI  (2403)
1 site
T T T A A A A A A T T T
PmeI  (2403)
1 site
G T T T A A A C C A A A T T T G
TspMI  (2424)
1 site
C C C G G G G G G C C C
XmaI  (2424)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2426)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
XbaI  (2435)
1 site
T C T A G A A G A T C T
SbfI  (2451)
1 site
C C T G C A G G G G A C G T C C
BlpI  (2516)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NotI  (2546)
1 site
G C G G C C G C C G C C G G C G
PsiI  (2669)
1 site
T T A T A A A A T A T T
MfeI  (2698)
1 site
C A A T T G G T T A A C
SfiI  (3229)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (3275)
1 site
A G G C C T T C C G G A
AvrII  (3276)
1 site
C C T A G G G G A T C C
PflMI  (3330)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
DraIII  (3530)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
hRluc
3342 .. 4274  =  933 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
hRluc
3342 .. 4274  =  933 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
NeoR/KanR
4326 .. 5117  =  792 bp
263 amino acids  =  28.9 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4326 .. 5117  =  792 bp
263 amino acids  =  28.9 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
GAL4 DNA binding domain
1083 .. 1520  =  438 bp
146 amino acids  =  16.8 kDa
Product: DNA binding domain of the GAL4 transcriptional activator
GAL4 DNA binding domain
1083 .. 1520  =  438 bp
146 amino acids  =  16.8 kDa
Product: DNA binding domain of the GAL4 transcriptional activator
IgA linker
1527 .. 1553  =  27 bp
9 amino acids  =  880.0 Da
Product: peptide derived from a human Ig alpha chain
IgA linker
1527 .. 1553  =  27 bp
9 amino acids  =  880.0 Da
Product: peptide derived from a human Ig alpha chain
GR-LBD
1563 .. 2399  =  837 bp
278 amino acids  =  32.0 kDa
Product: glucocorticoid nuclear receptor ligand binding domain
GR-LBD
1563 .. 2399  =  837 bp
278 amino acids  =  32.0 kDa
Product: glucocorticoid nuclear receptor ligand binding domain
AmpR
5490 .. 6350  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5490 .. 5558  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5490 .. 6350  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5559 .. 6350  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5490 .. 6350  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
6508 .. 7096  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6508 .. 7096  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
2934 .. 3291  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2934 .. 3291  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
214 .. 517  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
214 .. 517  =  304 bp
human cytomegalovirus immediate early enhancer
cer region
7212 .. 7495  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
7212 .. 7495  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
SV40 poly(A) signal
2568 .. 2689  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2568 .. 2689  =  122 bp
SV40 polyadenylation signal
AmpR promoter
5385 .. 5489  =  105 bp
AmpR promoter
5385 .. 5489  =  105 bp
poly(A) signal
5181 .. 5229  =  49 bp
synthetic polyadenylation signal
poly(A) signal
5181 .. 5229  =  49 bp
synthetic polyadenylation signal
T7 promoter
1033 .. 1051  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1033 .. 1051  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
3142 .. 3277  =  136 bp
SV40 origin of replication
SV40 ori
3142 .. 3277  =  136 bp
SV40 origin of replication
ORF:  4495 .. 4881  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  1083 .. 2399  =  1317 bp
ORF:  438 amino acids  =  50.1 kDa
ORF:  3342 .. 5117  =  1776 bp
ORF:  591 amino acids  =  66.0 kDa
ORF:  5490 .. 6350  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4993 .. 5298  =  306 bp
ORF:  101 amino acids  =  11.5 kDa
ORF:  3207 .. 3494  =  288 bp
ORF:  95 amino acids  =  10.2 kDa
ORF:  4632 .. 5168  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  1565 .. 2002  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  5954 .. 6220  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pBIND-GR.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.