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pCMV-CLuc 2

Control vector with a strong constitutive CMV promoter for expressing secreted Cypridina luciferase in mammalian cells.

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pCMV-CLuc 2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  New England Biolabs
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SgrDI (6903) SspI (6786) ScaI (6462) PvuI (6352) AhdI (5982) BstZ17I (4710) BstBI (4420) RsrII (4254) BssHII (4135) MscI (3820) PstI (3791) PluTI (3741) SfoI (3739) NarI (3738) KasI (3737) SmaI (3551) BmeT110I (3550) AvaI - BsoBI - TspMI - XmaI (3549) AvrII (3528) MfeI (161) Bpu10I (180) NruI (208) MluI (228) SpeI (249) NdeI (484) SnaBI (590) HindIII (889) Acc65I (895) KpnI (899) BamHI (907) BsgI (1050) AarI (1072) XcmI (1317) BstEII (1428) PshAI (1661) PmlI (1815) BmgBI (1838) EcoO109I - KflI - PpuMI (1927) Bsu36I (2479) NotI (2582) XbaI (2638) DraIII (3004) StuI (3527) pCMV-CLuc 2 6905 bp
SgrDI  (6903)
1 site
C G T C G A C G G C A G C T G C
SspI  (6786)
1 site
A A T A T T T T A T A A
ScaI  (6462)
1 site
A G T A C T T C A T G A
PvuI  (6352)
1 site
C G A T C G G C T A G C
AhdI  (5982)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstZ17I  (4710)
1 site
G T A T A C C A T A T G
BstBI  (4420)
1 site
T T C G A A A A G C T T
RsrII  (4254)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (4135)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MscI  (3820)
1 site
T G G C C A A C C G G T
PstI  (3791)
1 site
C T G C A G G A C G T C
PluTI  (3741)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3739)
1 site
G G C G C C C C G C G G
NarI  (3738)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3737)
1 site
G G C G C C C C G C G G
SmaI  (3551)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BmeT110I  (3550)
1 site
C Y C G R G G R G C Y C
AvaI  (3549)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (3549)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (3549)
1 site
C C C G G G G G G C C C
XmaI  (3549)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
AvrII  (3528)
1 site
C C T A G G G G A T C C
MfeI  (161)
1 site
C A A T T G G T T A A C
Bpu10I  (180)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NruI  (208)
1 site
T C G C G A A G C G C T
MluI  (228)
1 site
A C G C G T T G C G C A
SpeI  (249)
1 site
A C T A G T T G A T C A
NdeI  (484)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (590)
1 site
T A C G T A A T G C A T
HindIII  (889)
1 site
A A G C T T T T C G A A