Plasmid Files

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SpeI  (1) 1 site

AarI  (6556) 1 site

Efficient cleavage requires at least two copies of the AarI recognition sequence. Sticky ends from different AarI sites may not be compatible. After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.

AflII  (5909) 1 site

The sticky ends produced by AflII are hard to ligate.

DrdI  (5576) 1 site

Sticky ends from different DrdI sites may not be compatible.

MauBI  (4980) 1 site

NruI  (4907) 1 site

PvuI  (4353) 1 site

FspI  (4205) 1 site

AfeI  (4033) 1 site

SalI  (3873) 1 site

BstZ17I  (3868) 1 site

DraIII  (3578) 1 site

Sticky ends from different DraIII sites may not be compatible.

BsmBI  (3399) 1 site

Sticky ends from different BsmBI sites may not be compatible.

NdeI  (236) 1 site

Prolonged incubation with NdeI may lead to removal of additional nucleotides.

SnaBI  (342) 1 site

Eco53kI  (568) 1 site

SacI  (570) 1 site

BamHI  (634) 1 site

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

BbsI  (655) 1 site

Sticky ends from different BbsI sites may not be compatible. BbsI gradually loses activity when stored at -20°C.

Bpu10I  (808) 1 site

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site. Sticky ends from different Bpu10I sites may not be compatible.

BstXI  (999) 1 site

Sticky ends from different BstXI sites may not be compatible.

BspDI  (1070) 1 site

ClaI  (1070) 1 site

BsrDI  (1127) 1 site

Sticky ends from different BsrDI sites may not be compatible.

PstI  (1462) 1 site

NheI  (1534) 1 site

BmtI  (1538) 1 site

AleI  (1594) 1 site

BlpI  (1600) 1 site

Sticky ends from different BlpI sites may not be compatible.

Bsu36I  (1907) 1 site

Sticky ends from different Bsu36I sites may not be compatible.

KflI  (1962) 1 site

Sticky ends from different KflI sites may not be compatible.

PpuMI  (1962) 1 site

Sticky ends from different PpuMI sites may not be compatible.

NotI  (2309) 1 site

BseRI  (2874) 1 site

Sticky ends from different BseRI sites may not be compatible. BseRI quickly loses activity at 37°C. Prolonged incubation with BseRI may lead to degradation of the DNA.

AvrII  (2878) 1 site

PflFI  (3049) 1 site

The 1-base overhangs produced by PflFI may be hard to ligate. Sticky ends from different PflFI sites may not be compatible.

Tth111I  (3049) 1 site

The 1-base overhangs produced by Tth111I may be hard to ligate. Sticky ends from different Tth111I sites may not be compatible.

BsiWI  (3063) 1 site

BsiWI is typically used at 55°C, but is 50% active at 37°C.

RsrII  (3123) 1 site

Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible. For full activity, add fresh DTT.

BstEII  (3141) 1 site

Sticky ends from different BstEII sites may not be compatible. BstEII is typically used at 60°C, but is 50% active at 37°C.

EarI  (3166) 1 site

Efficient cleavage requires at least two copies of the EarI recognition sequence. Sticky ends from different EarI sites may not be compatible.

SacII  (3221) 1 site

Efficient cleavage requires at least two copies of the SacII recognition sequence.

hCluc 646 .. 2307  =  1662 bp 553 amino acids  =  61.5 kDa Product: secreted Cypridina luciferase human codon-optimized

hCluc 646 .. 2307  =  1662 bp 553 amino acids  =  61.5 kDa Product: secreted Cypridina luciferase human codon-optimized

AmpR 3910 .. 4770  =  861 bp 286 amino acids  =  31.6 kDa    Segment 2:      3910 .. 4701  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 3910 .. 4770  =  861 bp 286 amino acids  =  31.6 kDa    Segment 1:  signal sequence      4702 .. 4770  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 3910 .. 4770  =  861 bp 286 amino acids  =  31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

PuroR 3007 .. 3606  =  600 bp 199 amino acids  =  21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin

PuroR 3007 .. 3606  =  600 bp 199 amino acids  =  21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin

ori 5034 .. 5622  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 5034 .. 5622  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

SV40 promoter 2564 .. 2893  =  330 bp SV40 enhancer and early promoter

SV40 promoter 2564 .. 2893  =  330 bp SV40 enhancer and early promoter

CMV enhancer 63 .. 366  =  304 bp human cytomegalovirus immediate early enhancer

CMV enhancer 63 .. 366  =  304 bp human cytomegalovirus immediate early enhancer

CMV promoter 367 .. 570  =  204 bp human cytomegalovirus (CMV) immediate early promoter

CMV promoter 367 .. 570  =  204 bp human cytomegalovirus (CMV) immediate early promoter

SV40 poly(A) signal 3736 .. 3857  =  122 bp SV40 polyadenylation signal

SV40 poly(A) signal 3736 .. 3857  =  122 bp SV40 polyadenylation signal

bGH poly(A) signal 2330 .. 2441  =  112 bp bovine growth hormone polyadenylation signal

bGH poly(A) signal 2330 .. 2441  =  112 bp bovine growth hormone polyadenylation signal

pause site 6495 .. 6586  =  92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene

pause site 6495 .. 6586  =  92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene

EM7 promoter 2941 .. 2988  =  48 bp synthetic bacterial promoter

EM7 promoter 2941 .. 2988  =  48 bp synthetic bacterial promoter

T7 promoter 615 .. 633  =  19 bp promoter for bacteriophage T7 RNA polymerase

T7 promoter 615 .. 633  =  19 bp promoter for bacteriophage T7 RNA polymerase

lac operator 6364 .. 6380  =  17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator 6364 .. 6380  =  17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

SV40 ori 2744 .. 2879  =  136 bp SV40 origin of replication

SV40 ori 2744 .. 2879  =  136 bp SV40 origin of replication

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