Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
AflII (5909) 1 site
CTTAAGGAATTC
DrdI (5576) 1 site
GACNNNNNNGTCCTGNNNNNNCAG
Sticky ends from different DrdI sites may not be compatible.
MauBI (4980) 1 site
CGCGCGCGGCGCGCGC
NruI (4907) 1 site
TCGCGAAGCGCT
PvuI (4353) 1 site
CGATCGGCTAGC
FspI (4205) 1 site
TGCGCAACGCGT
AfeI (4033) 1 site
AGCGCTTCGCGA
SalI (3873) 1 site
GTCGACCAGCTG
BstZ17I (3868) 1 site
GTATACCATATG
DraIII (3578) 1 site
CACNNNGTGGTGNNNCAC
Sticky ends from different DraIII sites may not be compatible.
BsmBI (3399) 1 site
CGTCTCNGCAGAGN(N)4
Sticky ends from different BsmBI sites may not be compatible.
SpeI (1) 1 site
ACTAGTTGATCA
NdeI (236) 1 site
CATATGGTATAC
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI (342) 1 site
TACGTAATGCAT
Eco53kI (568) 1 site
GAGCTCCTCGAG
SacI (570) 1 site
GAGCTCCTCGAG
BamHI (634) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BbsI (655) 1 site
GAAGACNNCTTCTGNN(N)4
Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C.
Bpu10I (808) 1 site
CCTNAGCGGANTCG
Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible.
BstXI (999) 1 site
CCANNNNNNTGGGGTNNNNNNACC
Sticky ends from different BstXI sites may not be compatible.
BspDI (1070) 1 site
ATCGATTAGCTA
ClaI (1070) 1 site
ATCGATTAGCTA
BsrDI (1127) 1 site
GCAATGNNCGTTAC
Sticky ends from different BsrDI sites may not be compatible.
PstI (1462) 1 site
CTGCAGGACGTC
NheI (1534) 1 site
GCTAGCCGATCG
BmtI (1538) 1 site
GCTAGCCGATCG
AleI (1594) 1 site
CACNNNNGTGGTGNNNNCAC
BlpI (1600) 1 site
GCTNAGCCGANTCG
Sticky ends from different BlpI sites may not be compatible.
Bsu36I (1907) 1 site
CCTNAGGGGANTCC
Sticky ends from different Bsu36I sites may not be compatible.
KflI (1962) 1 site
GGGWCCCCCCWGGG
Sticky ends from different KflI sites may not be compatible.
PpuMI (1962) 1 site
RGGWCCYYCCWGGR
Sticky ends from different PpuMI sites may not be compatible.
NotI (2309) 1 site
GCGGCCGCCGCCGGCG
BseRI (2874) 1 site
GAGGAG(N)8NNCTCCTC(N)8
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA.
AvrII (2878) 1 site
CCTAGGGGATCC
PflFI (3049) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible.
Tth111I (3049) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible.
BsiWI (3063) 1 site
CGTACGGCATGC
BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII (3123) 1 site
CGGWCCGGCCWGGC
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
BstEII (3141) 1 site
GGTNACCCCANTGG
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C.