Resources
Plasmid Files

pCMV-Cypridina Luc

Control vector for constitutive high-level expression of secreted Cypridina luciferase under control of the CMV promoter.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCMV-Cypridina Luc Sequence and MappCMV-Cypridina Luc.dna
Map and Sequence File   
Sequence Author:  Thermo Scientific (Pierce)
Download Free Trial Get SnapGene Viewer

 SpeI (1) AarI (6556) AflII (5909) DrdI (5576) MauBI (4980) NruI (4907) PvuI (4353) FspI (4205) AfeI (4033) SalI (3873) BstZ17I (3868) DraIII (3578) BsmBI (3399) CMV enhancer NdeI (236) SnaBI (342) Eco53kI (568) SacI (570) BamHI (634) BbsI (655) Bpu10I (808) BstXI (999) BspDI - ClaI (1070) BsrDI (1127) PstI (1462) NheI (1534) BmtI (1538) AleI (1594) BlpI (1600) Bsu36I (1907) KflI - PpuMI (1962) NotI (2309) BseRI (2874) AvrII (2878) PflFI - Tth111I (3049) BsiWI (3063) RsrII (3123) BstEII (3141) EarI (3166) SacII (3221) pCMV-Cypridina Luc 6586 bp
SpeI  (1)
1 site
A C T A G T T G A T C A
AarI  (6556)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
AflII  (5909)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
DrdI  (5576)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
MauBI  (4980)
1 site
C G C G C G C G G C G C G C G C
NruI  (4907)
1 site
T C G C G A A G C G C T
PvuI  (4353)
1 site
C G A T C G G C T A G C
FspI  (4205)
1 site
T G C G C A A C G C G T
AfeI  (4033)
1 site
A G C G C T T C G C G A
SalI  (3873)
1 site
G T C G A C C A G C T G
BstZ17I  (3868)
1 site
G T A T A C C A T A T G
DraIII  (3578)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsmBI  (3399)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
NdeI  (236)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (342)
1 site
T A C G T A A T G C A T
Eco53kI  (568)
1 site
G A G C T C C T C G A G
SacI  (570)
1 site
G A G C T C C T C G A G
BamHI  (634)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
BbsI  (655)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bpu10I  (808)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstXI  (999)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BspDI  (1070)
1 site
A T C G A T T A G C T A
ClaI  (1070)
1 site
A T C G A T T A G C T A
BsrDI  (1127)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PstI  (1462)
1 site
C T G C A G G A C G T C
NheI  (1534)
1 site
G C T A G C C G A T C G
BmtI  (1538)
1 site
G C T A G C C G A T C G
AleI  (1594)
1 site
C A C N N N N G T G G T G N N N N C A C
BlpI  (1600)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Bsu36I  (1907)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
KflI  (1962)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1962)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
NotI  (2309)
1 site
G C G G C C G C C G C C G G C G
BseRI  (2874)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
AvrII  (2878)
1 site
C C T A G G G G A T C C
PflFI  (3049)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3049)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiWI  (3063)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (3123)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (3141)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
EarI  (3166)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SacII  (3221)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
hCluc
646 .. 2307  =  1662 bp
553 amino acids  =  61.5 kDa
Product: secreted Cypridina luciferase
human codon-optimized
hCluc
646 .. 2307  =  1662 bp
553 amino acids  =  61.5 kDa
Product: secreted Cypridina luciferase
human codon-optimized
AmpR
3910 .. 4770  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3910 .. 4701  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3910 .. 4770  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4702 .. 4770  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3910 .. 4770  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
PuroR
3007 .. 3606  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
3007 .. 3606  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
5034 .. 5622  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
5034 .. 5622  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
SV40 promoter
2564 .. 2893  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
2564 .. 2893  =  330 bp
SV40 enhancer and early promoter
CMV enhancer
63 .. 366  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
63 .. 366  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
367 .. 570  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
367 .. 570  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
3736 .. 3857  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3736 .. 3857  =  122 bp
SV40 polyadenylation signal
bGH poly(A) signal
2330 .. 2441  =  112 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
2330 .. 2441  =  112 bp
bovine growth hormone polyadenylation signal
pause site
6495 .. 6586  =  92 bp
RNA polymerase II transcriptional pause signal from
the human α2 globin gene
pause site
6495 .. 6586  =  92 bp
RNA polymerase II transcriptional pause signal from
the human α2 globin gene
EM7 promoter
2941 .. 2988  =  48 bp
synthetic bacterial promoter
EM7 promoter
2941 .. 2988  =  48 bp
synthetic bacterial promoter
T7 promoter
615 .. 633  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
615 .. 633  =  19 bp
promoter for bacteriophage T7 RNA polymerase
lac operator
6364 .. 6380  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
6364 .. 6380  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
SV40 ori
2744 .. 2879  =  136 bp
SV40 origin of replication
SV40 ori
2744 .. 2879  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter