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Plasmid Files

pGL2-Promoter

SV40 promoter-containing vector for measuring the activity of enhancer sequences with a luciferase assay.

To see this sequence with restriction sites, features, and translations, please download
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pGL2-Promoter.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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GLprimer1 (5757 .. 5779) DraIII (5232) XmnI (4679) ScaI (4560) EaeI (4468) BsaI (4141) AhdI (4080) AlwNI (3603) PciI (3187) AfeI (3063) RVprimer4 (2988 .. 3007) PshAI (3002) AccI (2938) SalI (2937) BamHI (2931) TspMI - XmaI (1) SmaI (3) Acc65I (8) KpnI (12) Eco53kI (16) SacI (18) MluI (22) NheI (28) BmtI (32) PaeR7I - XhoI (33) BglII (37) BtgI - NcoI (130) SfiI (176) StuI (222) AvrII (223) HindIII (239) GLprimer2 (269 .. 291) KasI (300) NarI (301) SfoI (302) PluTI (304) XbaI (315) PfoI * (375) BsiWI (422) BsrGI (758) EcoRI (855) BstEII (875) Bsu36I (881) SphI (931) XcmI (1003) EcoO109I - PpuMI (1447) PacI (1592) EcoRV (1606) BspDI - ClaI (1633) BfuAI - BspMI (1666) SgrAI (1696) EcoNI (1885) PflMI (2236) BsgI (2468) pGL2-Promoter 5790 bp
DraIII  (5232)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (4679)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (4560)
1 site
A G T A C T T C A T G A
EaeI  (4468)
1 site
Y G G C C R R C C G G Y
BsaI  (4141)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4080)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3603)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3187)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AfeI  (3063)
1 site
A G C G C T T C G C G A
PshAI  (3002)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AccI  (2938)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (2937)
1 site
G T C G A C C A G C T G
BamHI  (2931)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
TspMI  (1)
1 site
C C C G G G G G G C C C
XmaI  (1)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (3)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Acc65I  (8)
1 site
G G T A C C C C A T G G
KpnI  (12)
1 site
G G T A C C C C A T G G
Eco53kI  (16)
1 site
G A G C T C C T C G A G
SacI  (18)
1 site
G A G C T C C T C G A G
MluI  (22)
1 site
A C G C G T T G C G C A
NheI  (28)
1 site
G C T A G C C G A T C G
BmtI  (32)
1 site
G C T A G C C G A T C G
PaeR7I  (33)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (33)
1 site
C T C G A G G A G C T C
BglII  (37)
1 site
A G A T C T T C T A G A
BtgI  (130)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (130)
1 site
C C A T G G G G T A C C
SfiI  (176)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (222)
1 site
A G G C C T T C C G G A
AvrII  (223)
1 site
C C T A G G G G A T C C
HindIII  (239)
1 site
A A G C T T T T C G A A
KasI  (300)
1 site
G G C G C C C C G C G G
NarI  (301)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (302)
1 site
G G C G C C C C G C G G
PluTI  (304)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
XbaI  (315)
1 site
T C T A G A A G A T C T
PfoI  (375)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BsiWI  (422)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BsrGI  (758)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (855)
1 site
G A A T T C C T T A A G
BstEII  (875)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
Bsu36I  (881)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
SphI  (931)
1 site
G C A T G C C G T A