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pGL4.81[hRlucCP/Neo]

Promoterless vector encoding highly destabilized Renilla luciferase for measuring the activity of promoter and enhancer sequences.

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pGL4.81[hRlucCP Neo].dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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AarI (5031) RVprimer3 (5010 .. 5029) BsmBI (4858) SpeI (4845) BstZ17I (4526) SacII (4410) PvuI (4386) AhdI (4016) BstEII (3941) BstXI (3938) AleI (3936) NotI (3914) BspHI (3814) ApaLI (3408) BciVI (3297) AflIII - PciI (3094) RVprimer4 (2895 .. 2914) SalI (2844) BstBI (2830) AgeI (2765) BbvCI (2646) BglI - SfiI (8) Acc65I (14) KpnI (18) Eco53kI (23) SacI (25) NheI (27) BmtI (31) AbsI - PaeR7I - PspXI - XhoI (33) BglII (46) BglI - SfiI (59) HindIII (65) BglI (100) PfoI * (380) BsaI (640) NruI (761) ZraI (795) PflFI - Tth111I (796) AatII (797) PasI (874) EcoRI (1032) AflII (1062) AanI - AanI - PsiI (1359) HpaI (1379) MfeI (1388) BamHI (1481) FspI (1519) PvuII (1591) StuI (1914) PspOMI (2031) ApaI (2035) SgrAI (2177) EcoNI (2435) pGL4.81[hRlucCP/Neo] 5061 bp
AarI  (5031)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BsmBI  (4858)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
SpeI  (4845)
1 site
A C T A G T T G A T C A
BstZ17I  (4526)
1 site
G T A T A C C A T A T G
SacII  (4410)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuI  (4386)
1 site
C G A T C G G C T A G C
AhdI  (4016)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (3941)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (3938)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AleI  (3936)
1 site
C A C N N N N G T G G T G N N N N C A C
NotI  (3914)
1 site
G C G G C C G C C G C C G G C G
BspHI  (3814)
1 site
T C A T G A A G T A C T
ApaLI  (3408)
1 site
G T G C A C C A C G T G
BciVI  (3297)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
AflIII  (3094)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3094)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SalI  (2844)
1 site
G T C G A C C A G C T G
BstBI  (2830)
1 site
T T C G A A A A G C T T
AgeI  (2765)
1 site
A C C G G T T G G C C A
BbvCI  (2646)
1 site
C C T C A G C G G A G T C G
BglI  (8)
3 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (8)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
Acc65I  (14)
1 site
G G T A C C C C A T G G
KpnI  (18)
1 site
G G T A C C C C A T G G
Eco53kI  (23)
1 site
G A G C T C C T C G A G
SacI  (25)
1 site
G A G C T C C T C G A G
NheI  (27)
1 site
G C T A G C C G A T C G
BmtI  (31)
1 site
G C T A G C C G A T C G
AbsI  (33)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (33)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (33)
1 site
V C T C G A G B B G A G C T C V
XhoI  (33)
1 site
C T C G A G G A G C T C
BglII  (46)
1 site
A G A T C T T C T A G A
BglI  (59)
3 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (59)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
HindIII  (65)
1 site
A A G C T T T T C G A