pMCS-Gaussia Luc

Secreted Gaussia luciferase vector for cloning a transcriptional regulatory element.

Sequence Author: Thermo Fisher (Pierce)

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SpeI (1) PaqCI (4856) EcoRV (4562) ApaI (4222) PspOMI (4218) AflII (4209) DrdI (3876) MauBI (3280) ScaI (2763) FspI (2505) EcoRI (7) Acc65I (13) KpnI (17) Eco53kI (21) MCS SacI (23) PaeR7I - XhoI (25) HindIII (31) BamHI (37) BsaBI * (80) BbsI (126) NotI (609) AvrII (1178) TspMI - XmaI (1199) SmaI (1201) BclI * (1229) PflFI - Tth111I (1349) BsiWI (1363) RsrII (1423) BstEII (1441) EarI (1466) BsmBI - Esp3I (1699) DraIII (1878) NgoMIV (1979) NaeI (1981) BsmI (2116) BstZ17I (2168) AfeI (2333) pMCS-Gaussia Luc 4886 bp
SpeI  (1)
1 site
A C T A G T T G A T C A
PaqCI  (4856)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
EcoRV  (4562)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ApaI  (4222)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (4218)
1 site
G G G C C C C C C G G G
AflII  (4209)
1 site
C T T A A G G A A T T C
DrdI  (3876)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
MauBI  (3280)
1 site
C G C G C G C G G C G C G C G C
ScaI  (2763)
1 site
A G T A C T T C A T G A
FspI  (2505)
1 site
T G C G C A A C G C G T
EcoRI  (7)
1 site
G A A T T C C T T A A G
Acc65I  (13)
1 site
G G T A C C C C A T G G
KpnI  (17)
1 site
G G T A C C C C A T G G
Eco53kI  (21)
1 site
G A G C T C C T C G A G
SacI  (23)
1 site
G A G C T C C T C G A G
PaeR7I  (25)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (25)
1 site
C T C G A G G A G C T C
HindIII  (31)
1 site
A A G C T T T T C G A A
BamHI  (37)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsaBI  (80)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BbsI  (126)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
NotI  (609)
1 site
G C G G C C G C C G C C G G C G
AvrII  (1178)
1 site
C C T A G G G G A T C C
TspMI  (1199)
1 site
C C C G G G G G G C C C
XmaI  (1199)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1201)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BclI  (1229)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PflFI  (1349)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1349)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiWI  (1363)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (1423)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (1441)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
EarI  (1466)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BsmBI  (1699)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (1699)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
DraIII  (1878)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NgoMIV  (1979)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1981)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BsmI  (2116)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BstZ17I  (2168)
1 site
G T A T A C C A T A T G
AfeI  (2333)
1 site
A G C G C T T C G C G A
AmpR
2210 .. 3070  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2210 .. 3001  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2210 .. 3070  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3002 .. 3070  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2210 .. 3070  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
1307 .. 1906  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
1307 .. 1906  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
3334 .. 3922  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3334 .. 3922  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGLuc
50 .. 607  =  558 bp
185 amino acids  =  19.9 kDa
Product: secreted Gaussia luciferase
human codon-optimized
hGLuc
50 .. 607  =  558 bp
185 amino acids  =  19.9 kDa
Product: secreted Gaussia luciferase
human codon-optimized
SV40 promoter
864 .. 1193  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
864 .. 1193  =  330 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
2036 .. 2157  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2036 .. 2157  =  122 bp
SV40 polyadenylation signal
bGH poly(A) signal
630 .. 741  =  112 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
630 .. 741  =  112 bp
bovine growth hormone polyadenylation signal
pause site
4795 .. 4886  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
4795 .. 4886  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
EM7 promoter
1241 .. 1288  =  48 bp
synthetic bacterial promoter
EM7 promoter
1241 .. 1288  =  48 bp
synthetic bacterial promoter
MCS
1 .. 42  =  42 bp
multiple cloning site
MCS
1 .. 42  =  42 bp
multiple cloning site
lac operator
4664 .. 4680  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
4664 .. 4680  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
SV40 ori
1044 .. 1179  =  136 bp
SV40 origin of replication
SV40 ori
1044 .. 1179  =  136 bp
SV40 origin of replication
ORF:  50 .. 607  =  558 bp
ORF:  185 amino acids  =  19.9 kDa
ORF:  1307 .. 1906  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  2340 .. 2606  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  1467 .. 1793  =  327 bp
ORF:  108 amino acids  =  12.0 kDa
ORF:  2210 .. 3070  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4770 .. 318  =  435 bp
ORF:  144 amino acids  =  16.1 kDa
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