Resources
Plasmid Files

pMetLuc-Mem Reporter

In vivo imaging vector for measuring promoter activity with membrane-anchored Metridia luciferase.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pMetLuc-Mem Reporter Sequence and MappMetLuc-Mem Reporter.dna
Map and Sequence File   
Sequence Author:  Clontech
Download Free Trial Get SnapGene Viewer

 AfeI (14) pause site ScaI (4197) PfoI (2990) RsrII (2731) BsrDI (2448) PflFI - Tth111I (2333) FspI (2317) BglII (27) PaeR7I - XhoI (31) Eco53kI (36) SacI (38) HindIII (40) EcoRI (47) MCS PstI (56) SalI (57) AccI (58) Acc65I (63) KpnI (67) SacII (70) PspOMI (71) ApaI (75) BamHI (78) AgeI (84) ATG PmlI (123) BclI * (431) Bpu10I (598) BsrGI (632) XbaI * (941) MfeI (1037) HpaI (1050) Bts α I (1126) AflII (1169) DraIII (1403) SfiI (1990) BseRI (2033) StuI (2036) EagI (2121) pMetLuc-Mem Reporter 4351 bp
AfeI  (14)
1 site
A G C G C T T C G C G A
ScaI  (4197)
1 site
A G T A C T T C A T G A
PfoI  (2990)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2731)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2448)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2333)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2333)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2317)
1 site
T G C G C A A C G C G T
BglII  (27)
1 site
A G A T C T T C T A G A
PaeR7I  (31)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (31)
1 site
C T C G A G G A G C T C
Eco53kI  (36)
1 site
G A G C T C C T C G A G
SacI  (38)
1 site
G A G C T C C T C G A G
HindIII  (40)
1 site
A A G C T T T T C G A A
EcoRI  (47)
1 site
G A A T T C C T T A A G
PstI  (56)
1 site
C T G C A G G A C G T C
SalI  (57)
1 site
G T C G A C C A G C T G
AccI  (58)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (63)
1 site
G G T A C C C C A T G G
KpnI  (67)
1 site
G G T A C C C C A T G G
SacII  (70)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PspOMI  (71)
1 site
G G G C C C C C C G G G
ApaI  (75)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BamHI  (78)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
AgeI  (84)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
PmlI  (123)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BclI  (431)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Bpu10I  (598)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsrGI  (632)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
XbaI  (941)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (1037)
1 site
C A A T T G G T T A A C
HpaI  (1050)
1 site
G T T A A C C A A T T G
BtsαI  (1126)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
AflII  (1169)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
DraIII  (1403)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SfiI  (1990)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (2033)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
StuI  (2036)
1 site
A G G C C T T C C G G A
EagI  (2121)
1 site
C G G C C G G C C G G C
NeoR/KanR
2087 .. 2881  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
2087 .. 2881  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
MetLuc
322 .. 927  =  606 bp
202 amino acids  =  22.0 kDa
Product: Metridia luciferase
human codon-optimized
MetLuc
322 .. 927  =  606 bp
202 amino acids  =  22.0 kDa
Product: Metridia luciferase
human codon-optimized
ori
3489 .. 4077  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3489 .. 4077  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
1179 .. 1634  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
1179 .. 1634  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
1856 .. 2052  =  197 bp
SV40 early promoter
SV40 promoter
1856 .. 2052  =  197 bp
SV40 early promoter
TfR membrane anchor
103 .. 288  =  186 bp
62 amino acids  =  6.8 kDa
Product: signal-anchor region of the transferrin
receptor
TfR membrane anchor
103 .. 288  =  186 bp
62 amino acids  =  6.8 kDa
Product: signal-anchor region of the transferrin
receptor
SV40 poly(A) signal
1051 .. 1172  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1051 .. 1172  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1661 .. 1765  =  105 bp
AmpR promoter
1661 .. 1765  =  105 bp
pause site
4201 .. 4292  =  92 bp
RNA polymerase II transcriptional pause signal from
the human α2 globin gene
pause site
4201 .. 4292  =  92 bp
RNA polymerase II transcriptional pause signal from
the human α2 globin gene
MCS
12 .. 89  =  78 bp
multiple cloning site
MCS
12 .. 89  =  78 bp
multiple cloning site
poly(A) signal
4139 .. 4187  =  49 bp
synthetic polyadenylation signal
poly(A) signal
4139 .. 4187  =  49 bp
synthetic polyadenylation signal
HSV TK poly(A) signal
3113 .. 3160  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3113 .. 3160  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
ATG
97 .. 99  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
97 .. 99  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 ori
1903 .. 2038  =  136 bp
SV40 origin of replication
SV40 ori
1903 .. 2038  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter