Resources
Plasmid Files

pSF-CMV-RLuc

Mammalian vector for strong constitutive expression of Renilla luciferase.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pSF-CMV-RLuc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Oxford Genetics
Download Free Trial Get SnapGene Viewer


BtgI (5157) PmeI (5103) AhdI (4866) BsaI (4800) BpmI (4797) PmeI (3982) PpuMI - SanDI (3916) AscI (3825) FseI (3679) NaeI (3677) NgoMIV (3675) SwaI (3573) BspHI (3404) AlwNI (3100) DrdI (2792) SwaI (2680) AsiSI - PvuI (5) BglII (232) SnaBI (566) BglII (816) EagI - NotI (858) HindIII (869) Eco53kI (879) BanII - SacI (881) EcoRI (885) Acc65I (897) KpnI (901) BstAPI (1095) AanI (1199) BfuAI - BspMI (1241) Bpu10I (1555) BseRI - BsgI (1844) BspDI - ClaI (1881) BamHI (1890) StuI (1900) NheI (1906) BmtI (1910) AanI (2056) HpaI (2076) MfeI (2085) PstI - SbfI (2422) PacI (2554) pSF-CMV-RLuc 5222 bp
BtgI  (5157)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
PmeI  (5103)
2 sites
G T T T A A A C C A A A T T T G
AhdI  (4866)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (4800)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BpmI  (4797)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PmeI  (3982)
2 sites
G T T T A A A C C A A A T T T G
PpuMI  (3916)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (3916)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (3825)
1 site
G G C G C G C C C C G C G C G G
FseI  (3679)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
NaeI  (3677)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3675)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SwaI  (3573)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BspHI  (3404)
1 site
T C A T G A A G T A C T
AlwNI  (3100)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
DrdI  (2792)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
SwaI  (2680)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
AsiSI  (5)
1 site
G C G A T C G C C G C T A G C G
PvuI  (5)
1 site
C G A T C G G C T A G C
BglII  (232)
2 sites
A G A T C T T C T A G A
SnaBI  (566)
1 site
T A C G T A A T G C A T
BglII  (816)
2 sites
A G A T C T T C T A G A
EagI  (858)
1 site
C G G C C G G C C G G C
NotI  (858)
1 site
G C G G C C G C C G C C G G C G
HindIII  (869)
1 site
A A G C T T T T C G A A
Eco53kI  (879)
1 site
G A G C T C C T C G A G
BanII  (881)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (881)
1 site
G A G C T C C T C G A G
EcoRI  (885)
1 site
G A A T T C C T T A A G
Acc65I  (897)
1 site
G G T A C C C C A T G G
KpnI  (901)
1 site
G G T A C C C C A T G G
BstAPI  (1095)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
AanI  (1199)
2 sites
T T A T A A A A T A T T
BfuAI  (1241)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4