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Plasmid Files

pSF-CMVe-RLuc

Vector encoding the CMV enhancer and Renilla luciferase, for measuring the activity of promoters.

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pSF-CMVe-RLuc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Oxford Genetics
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PmeI (5079) RsrII (4772) BsrDI (4489) PflFI - Tth111I (4374) PmeI (4031) PpuMI - SanDI (3965) AscI (3874) FseI (3728) NaeI (3726) NgoMIV (3724) SwaI (3622) BspHI (3453) AlwNI (3149) SwaI (2729) PacI (2603) AsiSI - PvuI (5) BglII (232) AseI (244) SnaBI (577) NruI (710) PshAI (716) ScaI (728) AarI (793) SalI (824) AccI (825) SpeI (832) TspMI - XmaI (840) SmaI (842) PspOMI (848) ApaI (852) BstBI (857) BglII (865) EagI - NotI (907) HindIII (918) Eco53kI (928) SacI (930) EcoRI (934) Acc65I (946) KpnI (950) BstAPI (1144) AanI (1248) Bpu10I (1604) BseRI - BsgI (1893) BspDI - ClaI (1930) BamHI (1939) StuI (1949) NheI (1955) BmtI (1959) AanI (2105) HpaI (2125) MfeI (2134) PstI - SbfI (2471) BstEII (2577) pSF-CMVe-RLuc 5198 bp
PmeI  (5079)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (4772)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (4489)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (4374)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4374)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (4031)
2 sites
G T T T A A A C C A A A T T T G
PpuMI  (3965)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (3965)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (3874)
1 site
G G C G C G C C C C G C G C G G
FseI  (3728)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
NaeI  (3726)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3724)
1 site
G C C G G C C G G C