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Plasmid Files

pSF-PromMCS-FLuc

Vector for measuring promoter activity using firefly luciferase.

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pSF-PromMCS-FLuc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Oxford Genetics
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PmeI (5305) RsrII (4998) BsrDI (4715) PflFI - Tth111I (4600) PmeI (4257) PpuMI - SanDI (4191) AscI - BssHII (4100) FseI (3954) SwaI (3848) AlwNI (3375) ApaLI (3273) SwaI (2955) PacI (2829) BstEII (2803) AsiSI - PvuI (5) BglII (232) AarI (306) SalI (337) AccI (338) SpeI (345) TspMI - XmaI (353) SmaI (355) PspOMI (361) ApaI (365) BstBI (370) BglII (377) EagI - NotI (419) HindIII (430) Eco53kI (440) SacI (442) EcoRI (446) Acc65I (458) KpnI (462) NcoI (466) BbsI (526) PvuII (542) BsrGI (958) AleI (966) FspI (1290) KasI (1415) NarI (1416) SfoI (1417) PluTI (1419) BlpI (1422) AgeI (1599) DraIII (1614) SexAI * (1807) BpmI (1848) XbaI (2116) BseRI - BsgI (2119) BspDI - ClaI (2156) BamHI (2165) StuI (2175) NheI (2181) BmtI (2185) AanI - PsiI (2331) MfeI (2360) BglI (2520) PstI - SbfI (2697) pSF-PromMCS-FLuc 5424 bp
PmeI  (5305)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (4998)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (4715)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (4600)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4600)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (4257)
2 sites
G T T T A A A C C A A A T T T G
PpuMI  (4191)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (4191)
1 site
G G G W C C