Resources
Plasmid Files

pNGS FOS (linearized)

Linearized and dephosphorylated fosmid vector, for stable cloning of large inserts during the creation of genomic libraries for next-generation sequencing. 

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pNGS FOS (linearized) Sequence and MappNGS FOS (linearized).dna
Map and Sequence File   
Sequence Author:  Lucigen
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 < PmlI > (7738) Read 1 Primer (7703 .. 7735) Illumina Primer A (7678 .. 7697) 454 Primer A (7648 .. 7677) NotI (7642) SL1 (7594 .. 7614) < PmlI > (0) Read 2 Primer (4 .. 40) Illumina Primer B (44 .. 64) 454 Primer B (75 .. 94) NotI (95) SR4 (132 .. 152) pNGS FOS 7738 bp
End  (7738)
0 sites
NotI  (7642)
2 sites
G C G G C C G C C G C C G G C G
Start  (0)
0 sites
NotI  (95)
2 sites
G C G G C C G C C G C C G G C G
Read 1 Primer
33-mer  /  52% GC
1 binding site
7703 .. 7735  =  33 annealed bases
Tm  =  68°C
Illumina Primer A
20-mer  /  55% GC
1 binding site
7678 .. 7697  =  20 annealed bases
Tm  =  60°C
454 Primer A
30-mer  /  60% GC
1 binding site
7648 .. 7677  =  30 annealed bases
Tm  =  68°C
SL1
21-mer  /  57% GC
1 binding site
7594 .. 7614  =  21 annealed bases
Tm  =  59°C
Read 2 Primer
37-mer  /  59% GC
1 binding site
4 .. 40  =  37 annealed bases
Tm  =  74°C
Illumina Primer B
21-mer  /  52% GC
1 binding site
44 .. 64  =  21 annealed bases
Tm  =  58°C
454 Primer B
20-mer  /  60% GC
1 binding site
75 .. 94  =  20 annealed bases
Tm  =  59°C
SR4
21-mer  /  33% GC
1 binding site
132 .. 152  =  21 annealed bases
Tm  =  52°C
sopA
3259 .. 4434  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
sopA
3259 .. 4434  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
repE
5013 .. 5768  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
repE
5013 .. 5768  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
CmR
302 .. 961  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
302 .. 961  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
oriV
6154 .. 6768  =  615 bp
origin of replication for the bacterial F plasmid
oriV
6154 .. 6768  =  615 bp
origin of replication for the bacterial F plasmid
sopC
1742 .. 2215  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
sopC
1742 .. 2215  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
cos
1083 .. 1481  =  399 bp
lambda cos site; allows packaging into phage
lambda particles
cos
1083 .. 1481  =  399 bp
lambda cos site; allows packaging into phage
lambda particles
incC
4760 .. 5010  =  251 bp
incompatibility region of the bacterial F plasmid
incC
4760 .. 5010  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
5859 .. 6078  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
ori2
5859 .. 6078  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
cat promoter
199 .. 301  =  103 bp
promoter of the E. coli cat gene
cat promoter
199 .. 301  =  103 bp
promoter of the E. coli cat gene
rrnB T1 terminator
150 .. 195  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
150 .. 195  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
loxP
1032 .. 1065  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
1032 .. 1065  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
tonB terminator
975 .. 1006  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
tonB terminator
975 .. 1006  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
T7Te terminator
7535 .. 7562  =  28 bp
phage T7 early transcription terminator
T7Te terminator
7535 .. 7562  =  28 bp
phage T7 early transcription terminator
T7 promoter
108 .. 126  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
108 .. 126  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
7621 .. 7639  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
7621 .. 7639  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
sopB
2288 .. 3259  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
sopB
2288 .. 3259  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
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