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Plasmid Files

pRANGER™-BTB-5

Broad host range expression vector with a trimethoprim resistance marker and with the pBBR1 replicon for Gram-negative bacteria.

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pRANGER-BTB-5.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Lucigen
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HincII (0) EcoRI (3153) BsaHI (3040) MluI (2982) TsoI (2930) BstEII (2924) AgeI (2921) BspQI - EarI - SapI (2910) DrdI (2881) BspEI (2562) NruI (2555) SspI (2483) TatI (2266) NspI (2215) EciI (2189) AflII (2148) BsiHKAI (2104) BsaBI (2088) DraI (2026) BsgI (1830) BsiWI (1717) BtgZI (1659) EcoRI (4) HindIII (10) BpmI (26) SacII (103) PvuII (818) FspI (826) MscI (875) BmrI (934) BbsI (1061) AfeI (1127) BclI * (1255) BseRI (1370) AlwNI - BstAPI (1450) PshAI (1511) SnaBI (1537) pRANGER™-BTB-5 3161 bp
HincII  (0)
1 site
G T Y R A C C A R Y T G
EcoRI  (3153)
2 sites
G A A T T C C T T A A G
BsaHI  (3040)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
MluI  (2982)
1 site
A C G C G T T G C G C A
TsoI  (2930)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BstEII  (2924)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AgeI  (2921)
1 site
A C C G G T T G G C C A
BspQI  (2910)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (2910)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (2910)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
DrdI  (2881)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BspEI  (2562)
1 site
T C C G G A A G G C C T
NruI  (2555)
1 site
T C G C G A A G C G C T
SspI  (2483)
1 site
A A T A T T T T A T A A
TatI  (2266)
1 site
W G T A C W W C A T G W
NspI  (2215)
1 site
R C A T G Y Y G T A C R
EciI  (2189)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
AflII  (2148)
1 site
C T T A A G G A A T T C
BsiHKAI  (2104)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BsaBI  (2088)
1 site
G A T N N N N A T C C T A N N N N T A G
DraI  (2026)
1 site
T T T A A A A A A T T T
BsgI  (1830)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsiWI  (1717)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BtgZI  (1659)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
EcoRI  (4)
2 sites
G A A T T C C T T A A G
HindIII  (10)
1 site
A A G C T T T T C G A A
BpmI  (26)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
SacII  (103)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuII  (818)
1 site
C A G C T G G T C G A C
FspI  (826)
1 site
T G C G C A A C G C G T
MscI  (875)
1 site
T G G C C A A C C G G T
BmrI  (934)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BbsI  (1061)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AfeI  (1127)
1 site
A G C G C T T C G C G A
BclI  (1255)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BseRI  (1370)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AlwNI  (1450)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BstAPI  (1450)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PshAI  (1511)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SnaBI  (1537)
1 site
T A C G T A A T G C A T
araC
1943 .. 2821  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
araC
1943 .. 2821  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
pBBR1 Rep
872 .. 1534  =  663 bp
220 amino acids  =  24.4 kDa
Product: replication protein from the broad host range plasmid pBBR1
pBBR1 Rep
872 .. 1534  =  663 bp
220 amino acids  =  24.4 kDa
Product: replication protein from the broad host range plasmid pBBR1
TpR
1631 .. 1867  =  237 bp
78 amino acids  =  8.2 kDa
Product: E. coli plasmid-associated dihydrofolate reductase
confers resistance to trimethoprim
TpR
1631 .. 1867  =  237 bp
78 amino acids  =  8.2 kDa
Product: E. coli plasmid-associated dihydrofolate reductase
confers resistance to trimethoprim
araBAD promoter
2967 .. 3132  =  166 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction
araBAD promoter
2967 .. 3132  =  166 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction
soxR terminator
60 .. 96  =  37 bp
bidirectional E. coli soxR transcription terminator
soxR terminator
60 .. 96  =  37 bp
bidirectional E. coli soxR transcription terminator
tonB terminator
1884 .. 1915  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
1884 .. 1915  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
MCS
3153 .. 15  =  24 bp
multiple cloning site
MCS
3153 .. 15  =  24 bp
multiple cloning site
ORF:  422 .. 766  =  345 bp
ORF:  114 amino acids  =  12.2 kDa
ORF:  860 .. 1534  =  675 bp
ORF:  224 amino acids  =  24.9 kDa
ORF:  1631 .. 1867  =  237 bp
ORF:  78 amino acids  =  8.2 kDa
ORF:  2507 .. 2755  =  249 bp
ORF:  82 amino acids  =  9.3 kDa
ORF:  159 .. 398  =  240 bp
ORF:  79 amino acids  =  9.0 kDa
ORF:  1185 .. 1412  =  228 bp
ORF:  75 amino acids  =  8.3 kDa
ORF:  473 .. 712  =  240 bp
ORF:  79 amino acids  =  8.4 kDa
ORF:  776 .. 1291  =  516 bp
ORF:  171 amino acids  =  18.6 kDa
ORF:  1337 .. 1561  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  1943 .. 2872  =  930 bp
ORF:  309 amino acids  =  35.2 kDa
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