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Plasmid Files

pSMART® BAC v2.0 (EcoRI linearized)

EcoRI-linearized and dephosphorylated BAC vector with inducible copy number, suitable for cloning toxic inserts.

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pSMART BAC v2.0 (EcoRI linearized) Sequence and MappSMART BAC v2.0 (EcoRI linearized).dna
Map and Sequence File   
Sequence Author:  Lucigen
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 < EcoRI > (7592) PmlI (7588) AhdI (7580) NotI (7568) SL1 (7520 .. 7540) < EcoRI > (0) PmlI (7) AhdI (15) NotI (22) SR4 (58 .. 78) pSMART® BAC v2.0 7592 bp
End  (7592)
0 sites
PmlI  (7588)
2 sites
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (7580)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (7568)
2 sites
G C G G C C G C C G C C G G C G
Start  (0)
0 sites
PmlI  (7)
2 sites
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (15)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (22)
2 sites
G C G G C C G C C G C C G G C G
SL1
21-mer  /  57% GC
1 binding site
7520 .. 7540  =  21 annealed bases
Tm  =  59°C
SR4
21-mer  /  33% GC
1 binding site
58 .. 78  =  21 annealed bases
Tm  =  52°C
sopA
3185 .. 4360  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
sopA
3185 .. 4360  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
repE
4939 .. 5694  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
repE
4939 .. 5694  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
CmR
228 .. 887  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
228 .. 887  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
oriV
6080 .. 6694  =  615 bp
origin of replication for the bacterial F plasmid
oriV
6080 .. 6694  =  615 bp
origin of replication for the bacterial F plasmid
sopC
1668 .. 2141  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
sopC
1668 .. 2141  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
cos
1009 .. 1408  =  400 bp
lambda cos site; allows packaging into phage
lambda particles
cos
1009 .. 1408  =  400 bp
lambda cos site; allows packaging into phage
lambda particles
incC
4686 .. 4936  =  251 bp
incompatibility region of the bacterial F plasmid
incC
4686 .. 4936  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
5785 .. 6004  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
ori2
5785 .. 6004  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
cat promoter
125 .. 227  =  103 bp
promoter of the E. coli cat gene
cat promoter
125 .. 227  =  103 bp
promoter of the E. coli cat gene
rrnBT1 terminator
76 .. 121  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnBT1 terminator
76 .. 121  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
loxP
958 .. 991  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
958 .. 991  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
tonB terminator
901 .. 932  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
tonB terminator
901 .. 932  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
T7Te terminator
7461 .. 7488  =  28 bp
phage T7 early transcription terminator
T7Te terminator
7461 .. 7488  =  28 bp
phage T7 early transcription terminator
T7 promoter
35 .. 53  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
35 .. 53  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
7547 .. 7565  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
7547 .. 7565  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
sopB
2214 .. 3185  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
sopB
2214 .. 3185  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
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