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Plasmid Files

pSMART® BAC v2.0 (HindIII linearized)

HindIII-linearized and dephosphorylated BAC vector with inducible copy number, suitable for cloning toxic inserts.

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pSMART BAC v2.0 (HindIII linearized) Sequence and MappSMART BAC v2.0 (HindIII linearized).dna
Map and Sequence File   
Sequence Author:  Lucigen
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 < HindIII > (7606) EcoRI (7599) PmlI (7595) AhdI (7587) NotI (7575) SL1 (7527 .. 7547) < HindIII > (0) EcoRI (7) PmlI (14) AhdI (22) NotI (29) SR4 (65 .. 85) pSMART® BAC v2.0 7606 bp
End  (7606)
0 sites
EcoRI  (7599)
2 sites
G A A T T C C T T A A G
PmlI  (7595)
2 sites
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (7587)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (7575)
2 sites
G C G G C C G C C G C C G G C G
Start  (0)
0 sites
EcoRI  (7)
2 sites
G A A T T C C T T A A G
PmlI  (14)
2 sites
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (22)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (29)
2 sites
G C G G C C G C C G C C G G C G
SL1
21-mer  /  57% GC
1 binding site
7527 .. 7547  =  21 annealed bases
Tm  =  59°C
SR4
21-mer  /  33% GC
1 binding site
65 .. 85  =  21 annealed bases
Tm  =  52°C
sopA
3192 .. 4367  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
sopA
3192 .. 4367  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
repE
4946 .. 5701  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
repE
4946 .. 5701  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
CmR
235 .. 894  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
235 .. 894  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
oriV
6087 .. 6701  =  615 bp
origin of replication for the bacterial F plasmid
oriV
6087 .. 6701  =  615 bp
origin of replication for the bacterial F plasmid
sopC
1675 .. 2148  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
sopC
1675 .. 2148  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
cos
1016 .. 1415  =  400 bp
lambda cos site; allows packaging into phage
lambda particles
cos
1016 .. 1415  =  400 bp
lambda cos site; allows packaging into phage
lambda particles
incC
4693 .. 4943  =  251 bp
incompatibility region of the bacterial F plasmid
incC
4693 .. 4943  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
5792 .. 6011  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
ori2
5792 .. 6011  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
cat promoter
132 .. 234  =  103 bp
promoter of the E. coli cat gene
cat promoter
132 .. 234  =  103 bp
promoter of the E. coli cat gene
rrnBT1 terminator
83 .. 128  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnBT1 terminator
83 .. 128  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
loxP
965 .. 998  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
965 .. 998  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
tonB terminator
908 .. 939  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
tonB terminator
908 .. 939  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
T7Te terminator
7468 .. 7495  =  28 bp
phage T7 early transcription terminator
T7Te terminator
7468 .. 7495  =  28 bp
phage T7 early transcription terminator
T7 promoter
42 .. 60  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
42 .. 60  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
7554 .. 7572  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
7554 .. 7572  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
sopB
2221 .. 3192  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
sopB
2221 .. 3192  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
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