pSMART GC LK (linearized)

Linearized and dephosphorylated low copy-number vector with 3'-C tails for transcription-free GC cloning of PCR products.

Sequence Author: Lucigen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Lucigen Vectors | More Plasmid Sets
No matches
EcoRI (1979) EcoRV (1969) SL1 Primer (1929 .. 1949) AcuI (1764) AlwNI (1632) BsiHKAI (1534) ApaLI (1530) PspFI (1524) BseYI (1520) HaeII (1464) BciVI (1419) ApaI (1222) EcoO109I - PspOMI (1218) PvuII (1174) XmnI (1141) EcoRI (10) XbaI (16) EcoRV (22) DraI - SwaI (43) SR2 Primer (33 .. 58) tonB terminator BsaAI (112) AvaI - BmeT110I - BsoBI - PaeR7I - PspXI - XhoI (235) NruI (294) EarI (450) TsoI (478) PasI (509) EcoNI (549) SspI (562) BsrFI (591) AsiSI - PvuI (637) BsmBI (653) AseI (836) TaqII (891) PflMI (900) pSMART® GC LK 1992 bp
EcoRI  (1979)
2 sites
G A A T T C C T T A A G
EcoRV  (1969)
2 sites
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AcuI  (1764)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (1632)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BsiHKAI  (1534)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (1530)
1 site
G T G C A C C A C G T G
PspFI  (1524)
1 site
C C C A G C G G G T C G
BseYI  (1520)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
HaeII  (1464)
1 site
R G C G C Y Y C G C G R
BciVI  (1419)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
ApaI  (1222)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
EcoO109I  (1218)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (1218)
1 site
G G G C C C C C C G G G
PvuII  (1174)
1 site
C A G C T G G T C G A C
XmnI  (1141)
1 site
G A A N N N N T T C C T T N N N N A A G
EcoRI  (10)
2 sites
G A A T T C C T T A A G
XbaI  (16)
1 site
T C T A G A A G A T C T
EcoRV  (22)
2 sites
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
DraI  (43)
1 site
T T T A A A A A A T T T
SwaI  (43)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BsaAI  (112)
1 site
Y A C G T R R T G C A Y
AvaI  (235)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (235)
1 site
C Y C G R G G R G C Y C
BsoBI  (235)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (235)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (235)
1 site
V C T C G A G B B G A G C T C V
XhoI  (235)
1 site
C T C G A G G A G C T C
NruI  (294)
1 site
T C G C G A A G C G C T
EarI  (450)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
TsoI  (478)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PasI  (509)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
EcoNI  (549)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SspI  (562)
1 site
A A T A T T T T A T A A
BsrFI  (591)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
AsiSI  (637)
1 site
G C G A T C G C C G C T A G C G
PvuI  (637)
1 site
C G A T C G G C T A G C
BsmBI  (653)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
AseI  (836)
1 site
A T T A A T T A A T T A
TaqII  (891)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
PflMI  (900)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
SL1 Primer
21-mer  /  57% GC
1 binding site
1929 .. 1949  =  21 annealed bases
Tm  =  59°C
SR2 Primer
26-mer  /  38% GC
1 binding site
33 .. 58  =  26 annealed bases
Tm  =  56°C
KanR
206 .. 1021  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
206 .. 1021  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
ori
1277 .. 1864  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1277 .. 1864  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
rop
1026 .. 1214  =  189 bp
62 amino acids  =  7.1 kDa
Product: Rop protein, which maintains plasmids at low copy number
rop
1026 .. 1214  =  189 bp
62 amino acids  =  7.1 kDa
Product: Rop protein, which maintains plasmids at low copy number
cat promoter
103 .. 205  =  103 bp
promoter of the E. coli cat gene
cat promoter
103 .. 205  =  103 bp
promoter of the E. coli cat gene
tonB terminator
71 .. 102  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
71 .. 102  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
T3Te terminator
1886 .. 1915  =  30 bp
phage T3 early transcription terminator
T3Te terminator
1886 .. 1915  =  30 bp
phage T3 early transcription terminator
T7Te terminator
1238 .. 1265  =  28 bp
phage T7 early transcription terminator
T7Te terminator
1238 .. 1265  =  28 bp
phage T7 early transcription terminator
ORF:  1117 .. 1602  =  486 bp
ORF:  161 amino acids  =  18.0 kDa
ORF:  206 .. 1021  =  816 bp
ORF:  271 amino acids  =  30.9 kDa
ORF:  1080 .. 1307  =  228 bp
ORF:  75 amino acids  =  8.6 kDa
Click here to try SnapGene

Download pSMART GC LK (linearized).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.