Resources
Plasmid Files

pCI-neo

Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCI-neo Sequence and MappCI-neo.dna
Map and Sequence File   
Sequence Author:  Promega
Download Free Trial Get SnapGene Viewer

 BglII (5467) AlwNI (5046) AhdI (4567) BpmI (4498) XmnI (3967) EcoO109I (3587) PfoI (3528) BamHI (3385) BstXI (3312) BstBI (3273) RsrII (3107) BssHII (2988) PflFI - Tth111I (2709) BsrGI (96) SpeI (152) NdeI (387) SnaBI (493) AsiSI (664) minimal CMV promoter Eco53kI (727) SacI (729) I-PpoI (851) BbsI (961) NheI (1085) BmtI (1089) PaeR7I - XhoI (1091) EcoRI (1096) AflIII - MluI (1102) XbaI (1114) SalI (1120) AccI (1121) TspMI - XmaI (1125) SmaI (1127) NotI (1131) T3 promoter HpaI (1297) MfeI (1306) DraIII (1716) SexAI * (2164) SfiI (2350) BseRI (2393) StuI (2396) AvrII (2397) KasI (2590) NarI (2591) SfoI (2592) PluTI (2594) pCI-neo 5472 bp
BglII  (5467)
1 site
A G A T C T T C T A G A
AlwNI  (5046)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (4567)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BpmI  (4498)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
XmnI  (3967)
1 site
G A A N N N N T T C C T T N N N N A A G
EcoO109I  (3587)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (3528)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BamHI  (3385)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
BstXI  (3312)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BstBI  (3273)
1 site
T T C G A A A A G C T T
RsrII  (3107)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (2988)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PflFI  (2709)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2709)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsrGI  (96)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (152)
1 site
A C T A G T T G A T C A
NdeI  (387)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (493)
1 site
T A C G T A A T G C A T
AsiSI  (664)
1 site
G C G A T C G C C G C T A G C G
Eco53kI  (727)
1 site
G A G C T C C T C G A G
SacI  (729)
1 site
G A G C T C C T C G A G
I-PpoI  (851)
1 site
C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G

I-PpoI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
BbsI  (961)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
NheI  (1085)
1 site
G C T A G C C G A T C G
BmtI  (1089)
1 site
G C T A G C C G A T C G
PaeR7I  (1091)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1091)
1 site
C T C G A G G A G C T C
EcoRI  (1096)
1 site
G A A T T C C T T A A G
AflIII  (1102)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
MluI  (1102)
1 site
A C G C G T T G C G C A
XbaI  (1114)
1 site
T C T A G A A G A T C T
SalI  (1120)
1 site
G T C G A C C A G C T G
AccI  (1121)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
TspMI  (1125)
1 site
C C C G G G G G G C C C
XmaI  (1125)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1127)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
NotI  (1131)
1 site
G C G G C C G C C G C C G G C G
HpaI  (1297)
1 site
G T T A A C C A A T T G
MfeI  (1306)
1 site
C A A T T G G T T A A C
DraIII  (1716)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SexAI  (2164)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (2350)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (2393)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
StuI  (2396)
1 site
A G G C C T T C C G G A
AvrII  (2397)
1 site
C C T A G G G G A T C C
KasI  (2590)
1 site
G G C G C C C C G C G G
NarI  (2591)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (2592)
1 site
G G C G C C C C G C G G
PluTI  (2594)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
AmpR
3780 .. 4640  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   3780 .. 3848  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3780 .. 4640  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3849 .. 4640  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3780 .. 4640  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
NeoR/KanR
2463 .. 3257  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
2463 .. 3257  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
ori
4811 .. 5399  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4811 .. 5399  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
1483 .. 1938  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
1483 .. 1938  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
2055 .. 2412  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2055 .. 2412  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
213 .. 517  =  305 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
213 .. 517  =  305 bp
human cytomegalovirus immediate early enhancer
chimeric intron
890 .. 1022  =  133 bp
chimera between introns from human β-globin and
immunoglobulin heavy chain genes
chimeric intron
890 .. 1022  =  133 bp
chimera between introns from human β-globin and
immunoglobulin heavy chain genes
SV40 poly(A) signal
1176 .. 1297  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1176 .. 1297  =  122 bp
SV40 polyadenylation signal
AmpR promoter
3675 .. 3779  =  105 bp
AmpR promoter
3675 .. 3779  =  105 bp
MCS
1085 .. 1137  =  53 bp
multiple cloning site
MCS
1085 .. 1137  =  53 bp
multiple cloning site
poly(A) signal
3321 .. 3369  =  49 bp
synthetic polyadenylation signal
poly(A) signal
3321 .. 3369  =  49 bp
synthetic polyadenylation signal
minimal CMV promoter
691 .. 729  =  39 bp
human cytomegalovirus (CMV) immediate early
promoter
minimal CMV promoter
691 .. 729  =  39 bp
human cytomegalovirus (CMV) immediate early
promoter
T3 promoter
1141 .. 1158  =  18 bp
promoter for bacteriophage T3 RNA polymerase
(shorter by one base than the standard T3
promoter)
T3 promoter
1141 .. 1158  =  18 bp
promoter for bacteriophage T3 RNA polymerase
(shorter by one base than the standard T3
promoter)
SV40 ori
2263 .. 2398  =  136 bp
SV40 origin of replication
SV40 ori
2263 .. 2398  =  136 bp
SV40 origin of replication
T7 promoter
1067 .. 1085  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1067 .. 1085  =  19 bp
promoter for bacteriophage T7 RNA polymerase
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter