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Plasmid Files

pCMV-HA-C

Vector for expressing a C-terminally HA-tagged protein in mammalian cells.

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pCMV-HA-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PluTI (3613) SfoI (3611) NarI (3610) KasI (3609) BstAPI (3559) SspI (3191) XmnI (2986) ScaI (2867) NmeAIII (2535) BsaI (2448) AhdI (2387) AlwNI (1910) BfuAI - BspMI (12) SbfI (23) BtgZI (300) BsaAI - SnaBI (306) NcoI - StyI (326) Eco53kI (532) SacI (534) BbsI (600) BspEI (617) PpuMI (702) BseRI (730) StuI (769) PspOMI * (820) ApaI * (824) EcoRI (832) SalI (839) AccI (840) BglII (847) AvaI - BsoBI - PaeR7I - XhoI (853) BmeT110I (854) Acc65I (858) KpnI (862) AflII (892) EagI - NotI (898) PsiI (1022) MfeI (1051) NheI (1133) BmtI (1137) BspQI - SapI (1378) AflIII - PciI (1494) BseYI (1798) PspFI (1802) pCMV-HA-C 3769 bp
PluTI  (3613)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3611)
1 site
G G C G C C C C G C G G
NarI  (3610)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3609)
1 site
G G C G C C C C G C G G
BstAPI  (3559)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
SspI  (3191)
1 site
A A T A T T T T A T A A
XmnI  (2986)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2867)
1 site
A G T A C T T C A T G A
NmeAIII  (2535)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaI  (2448)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2387)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1910)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BfuAI  (12)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (12)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SbfI  (23)
1 site
C C T G C A G G G G A C G T C C
BtgZI  (300)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaAI  (306)
1 site
Y A C G T R R T G C