Resources
Plasmid Files

pCMV-Tag 5C

Mammalian expression vector for tagging proteins with a C-terminal Myc epitope. For other reading frames, use pCMV‑Tag 5A or pCMV‑Tag 5B.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCMV-Tag 5C Sequence and MappCMV-Tag 5C.dna
Map and Sequence File   
Sequence Author:  Agilent Technologies
Download Free Trial Get SnapGene Viewer

 PciI (4272) ApaLI (3958) BsaI (3343) PfoI (3129) BstBI (3036) RsrII (2870) BsrDI (2587) PflFI - Tth111I (2472) MscI (2436) PluTI (2357) SfoI (2355) NarI (2354) KasI (2353) StuI (2175) NdeI (240) SnaBI (346) NheI (597) BmtI (601) Eco53kI (653) SacI (655) AleI (661) SacII (662) BstXI (663) NotI (668) SrfI (682) BamHI (687) PstI (703) EcoRI (705) EcoRV (713) HindIII (717) SalI (732) AccI (733) PaeR7I - PspXI - XhoI (740) EarI (756) PspOMI (775) ApaI (779) PvuI (857) BclI * (1011) MfeI (1104) HpaI (1117) Bts α I (1193) MluI (1240) DraIII (1470) SfiI (2129) BseRI (2172) pCMV-Tag 5C 4324 bp
PciI  (4272)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (3958)
1 site
G T G C A C C A C G T G
BsaI  (3343)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3129)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (3036)
1 site
T T C G A A A A G C T T
RsrII  (2870)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2587)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2472)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2472)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (2436)
1 site
T G G C C A A C C G G T
PluTI  (2357)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (2355)
1 site
G G C G C C C C G C G G
NarI  (2354)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (2353)
1 site
G G C G C C C C G C G G
StuI  (2175)
1 site
A G G C C T T C C G G A
NdeI  (240)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (346)
1 site
T A C G T A A T G C A T
NheI  (597)
1 site
G C T A G C C G A T C G
BmtI  (601)
1 site
G C T A G C C G A T C G
Eco53kI  (653)
1 site
G A G C T C C T C G A G
SacI  (655)
1 site
G A G C T C C T C G A G
AleI  (661)
1 site
C A C N N N N G T G G T G N N N N C A C
SacII  (662)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BstXI  (663)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (668)
1 site
G C G G C C G C C G C C G G C G
SrfI  (682)
1 site
G C C C G G G C C G G G C C C G
BamHI  (687)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
PstI  (703)
1 site
C T G C A G G A C G T C
EcoRI  (705)
1 site
G A A T T C C T T A A G
EcoRV  (713)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
HindIII  (717)
1 site
A A G C T T T T C G A A
SalI  (732)
1 site
G T C G A C C A G C T G
AccI  (733)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PaeR7I  (740)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (740)
1 site
V C T C G A G B B G A G C T C V
XhoI  (740)
1 site
C T C G A G G A G C T C
EarI  (756)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
PspOMI  (775)
1 site
G G G C C C C C C G G G
ApaI  (779)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PvuI  (857)
1 site
C G A T C G G C T A G C
BclI  (1011)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1104)
1 site
C A A T T G G T T A A C
HpaI  (1117)
1 site
G T T A A C C A A T T G
BtsαI  (1193)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
MluI  (1240)
1 site
A C G C G T T G C G C A
DraIII  (1470)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SfiI  (2129)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (2172)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
NeoR/KanR
2226 .. 3020  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
2226 .. 3020  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
ori
3628 .. 4216  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3628 .. 4216  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
1246 .. 1701  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
1246 .. 1701  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
1834 .. 2191  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
1834 .. 2191  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
66 .. 370  =  305 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
66 .. 370  =  305 bp
human cytomegalovirus immediate early enhancer
CMV promoter
371 .. 574  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
371 .. 574  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
1118 .. 1239  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1118 .. 1239  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1728 .. 1830  =  103 bp
AmpR promoter
1728 .. 1830  =  103 bp
MCS
651 .. 745  =  95 bp
multiple cloning site
MCS
651 .. 745  =  95 bp
multiple cloning site
HSV TK poly(A) signal
3252 .. 3299  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3252 .. 3299  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
T3 promoter
620 .. 638  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T3 promoter
620 .. 638  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T7 promoter
826 .. 844  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
826 .. 844  =  19 bp
promoter for bacteriophage T7 RNA polymerase
stop codons
788 .. 798  =  11 bp
stop codons in all three reading frames
stop codons
788 .. 798  =  11 bp
stop codons in all three reading frames
Myc
743 .. 772  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
743 .. 772  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter