Plasmid Files

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.



AanI  (5733) 2 sites

DraIII  (5608) 1 site

Sticky ends from different DraIII sites may not be compatible.

ScaI  (4936) 1 site

AlwNI  (3979) 1 site

Sticky ends from different AlwNI sites may not be compatible.

PciI  (3563) 1 site

PciI is inhibited by nonionic detergents.

BstZ17I  (3184) 1 site

BsmI  (3132) 1 site

Sticky ends from different BsmI sites may not be compatible.

AanI  (3071) 2 sites

PfoI  (2987) 1 site

Sticky ends from different PfoI sites may not be compatible.

BstBI  (2894) 1 site

BsrGI  (301) 1 site

BsrGI is typically used at 37°C, but is even more active at 60°C.

NdeI  (592) 1 site

Prolonged incubation with NdeI may lead to removal of additional nucleotides.

SnaBI  (698) 1 site

Eco53kI  (924) 1 site

SacI  (926) 1 site

EcoRI  (979) 1 site

BamHI  (992) 1 site

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

Acc65I  (998) 1 site

KpnI  (1002) 1 site

AsiSI  (1024) 1 site

SgfI  (1024) 1 site

MreI  (1026) 1 site

SgrAI  (1026) 1 site

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.

AscI  (1030) 1 site

BssHII  (1030) 1 site

BssHII is typically used at 50°C, but is 75% active at 37°C.

HindIII  (1044) 1 site

RsrII  (1061) 1 site

Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible. For full activity, add fresh DTT.

MluI  (1067) 1 site

NotI  (1077) 1 site

PaeR7I  (1085) 1 site

PaeR7I does not recognize the sequence CTCTCGAG.

PspXI  (1085) 1 site

XhoI  (1085) 1 site

PmeI  (1094) 1 site

SacII  (1108) 1 site

Efficient cleavage requires at least two copies of the SacII recognition sequence.

AleI  (1194) 1 site

BbsI  (1317) 1 site

Sticky ends from different BbsI sites may not be compatible. BbsI gradually loses activity when stored at -20°C.

BbvCI  (1420) 1 site

Bpu10I  (1420) 1 site

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site. Sticky ends from different Bpu10I sites may not be compatible.

BsmBI  (1484) 1 site

Sticky ends from different BsmBI sites may not be compatible.

BstXI  (1571) 1 site

Sticky ends from different BstXI sites may not be compatible.

AgeI  (1682) 1 site

AgeI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

XcmI  (1777) 1 site

The 1-base overhangs produced by XcmI may be hard to ligate. Sticky ends from different XcmI sites may not be compatible.

SexAI  (1869) 1 site

* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible.

SfiI  (2055) 1 site

Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible.

StuI  (2101) 1 site

AvrII  (2102) 1 site

BclI  (2153) 1 site

* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C.

BsiWI  (2166) 1 site

BsiWI is typically used at 55°C, but is 50% active at 37°C.

KasI  (2322) 1 site

NarI  (2323) 1 site

Efficient cleavage requires at least two copies of the NarI recognition sequence.

SfoI  (2324) 1 site

PluTI  (2326) 1 site

Efficient cleavage requires at least two copies of the PluTI recognition sequence.

AarI  (2626) 1 site

Efficient cleavage requires at least two copies of the AarI recognition sequence. Sticky ends from different AarI sites may not be compatible. After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.

BsaBI  (2638) 1 site

* Blocked by Dam methylation.

PspOMI  (2759) 1 site

ApaI  (2763) 1 site

ApaI can be used between 25°C and 37°C.

PstI  (2878) 1 site

VP1.5 (forward primer) 18-mer  /  44% GC 1 binding site 839 .. 856  =  18 annealed bases Tm  =  51°C

XL39 (reverse primer) 20-mer  /  55% GC 1 binding site 1200 .. 1219  =  20 annealed bases Tm  =  59°C

AmpR 4383 .. 5243  =  861 bp 286 amino acids  =  31.6 kDa    Segment 2:      4383 .. 5174  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 4383 .. 5243  =  861 bp 286 amino acids  =  31.6 kDa    Segment 1:  signal sequence      5175 .. 5243  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 4383 .. 5243  =  861 bp 286 amino acids  =  31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

TurboGFP 2175 .. 2873  =  699 bp 232 amino acids  =  25.7 kDa Product: improved green fluorescent protein from Pontellina plumata (Evdokimov et al., 2006) mammalian codon-optimized

TurboGFP 2175 .. 2873  =  699 bp 232 amino acids  =  25.7 kDa Product: improved green fluorescent protein from Pontellina plumata (Evdokimov et al., 2006) mammalian codon-optimized

hGH poly(A) signal 1137 .. 1759  =  623 bp human growth hormone polyadenylation signal

hGH poly(A) signal 1137 .. 1759  =  623 bp human growth hormone polyadenylation signal

ori 3624 .. 4212  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 3624 .. 4212  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

f1 ori 5375 .. 25  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

f1 ori 5375 .. 25  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

CMV enhancer 343 .. 722  =  380 bp human cytomegalovirus immediate early enhancer

CMV enhancer 343 .. 722  =  380 bp human cytomegalovirus immediate early enhancer

SV40 promoter 1788 .. 2117  =  330 bp SV40 enhancer and early promoter

SV40 promoter 1788 .. 2117  =  330 bp SV40 enhancer and early promoter

CMV promoter 723 .. 926  =  204 bp human cytomegalovirus (CMV) immediate early promoter

CMV promoter 723 .. 926  =  204 bp human cytomegalovirus (CMV) immediate early promoter

MCS 979 .. 1110  =  132 bp multiple cloning site

MCS 979 .. 1110  =  132 bp multiple cloning site

SV40 poly(A) signal 3052 .. 3173  =  122 bp SV40 polyadenylation signal

SV40 poly(A) signal 3052 .. 3173  =  122 bp SV40 polyadenylation signal

AmpR promoter 5244 .. 5348  =  105 bp

AmpR promoter 5244 .. 5348  =  105 bp

T7 promoter 952 .. 970  =  19 bp promoter for bacteriophage T7 RNA polymerase

T7 promoter 952 .. 970  =  19 bp promoter for bacteriophage T7 RNA polymerase

SV40 ori 1968 .. 2103  =  136 bp SV40 origin of replication

SV40 ori 1968 .. 2103  =  136 bp SV40 origin of replication

Try SnapGene and create your own beautiful maps

SnapGene®	SnapGene® Viewer
SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures. Priced accessibly so that everyone in your lab can have a license. Learn More...	SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats. Free! Because there should be no barriers to seeing your data. Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

hidden link