Resources
Plasmid Files

pCMV6-A-GFP

PrecisionShuttle™ mammalian expression vector for coexpressing an untagged ORF with a TurboGFP marker.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCMV6-A-GFP Sequence and MappCMV6-A-GFP.dna
Map and Sequence File   
Sequence Author:  OriGene
Download Free Trial Get SnapGene Viewer

 AanI (5733) DraIII (5608) ScaI (4936) AlwNI (3979) PciI (3563) BstZ17I (3184) BsmI (3132) AanI (3071) PfoI (2987) BstBI (2894) BsrGI (301) CMV enhancer NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI - BssHII (1030) HindIII (1044) RsrII (1061) MluI (1067) NotI (1077) PaeR7I - PspXI - XhoI (1085) PmeI (1094) SacII (1108) AleI (1194) XL39 (reverse primer) (1200 .. 1219) BbsI (1317) BbvCI - Bpu10I (1420) BsmBI (1484) BstXI (1571) AgeI (1682) XcmI (1777) SexAI * (1869) SfiI (2055) StuI (2101) AvrII (2102) BclI * (2153) BsiWI (2166) KasI (2322) NarI (2323) SfoI (2324) PluTI (2326) AarI (2626) BsaBI * (2638) PspOMI (2759) ApaI (2763) PstI (2878) pCMV6-A-GFP 5805 bp
AanI  (5733)
2 sites
T T A T A A A A T A T T
DraIII  (5608)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (4936)
1 site
A G T A C T T C A T G A
AlwNI  (3979)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3563)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3184)
1 site
G T A T A C C A T A T G
BsmI  (3132)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3071)
2 sites
T T A T A A A A T A T T
PfoI  (2987)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (2894)
1 site
T T C G A A A A G C T T
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C A T G G
AsiSI  (1024)
1 site
G C G A T C G C C G C T A G C G
SgfI  (1024)
1 site
G C G A T C G C C G C T A G C G
MreI  (1026)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (1026)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AscI  (1030)
1 site
G G C G C G C C C C G C G C G G
BssHII  (1030)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
HindIII  (1044)
1 site
A A G C T T T T C G A A
RsrII  (1061)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (1067)
1 site
A C G C G T T G C G C A
NotI  (1077)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (1085)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1085)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1085)
1 site
C T C G A G G A G C T C
PmeI  (1094)
1 site
G T T T A A A C C A A A T T T G
SacII  (1108)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
AleI  (1194)
1 site
C A C N N N N G T G G T G N N N N C A C
BbsI  (1317)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbvCI  (1420)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1420)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (1484)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BstXI  (1571)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AgeI  (1682)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
XcmI  (1777)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SexAI  (1869)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (2055)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (2101)
1 site
A G G C C T T C C G G A
AvrII  (2102)
1 site
C C T A G G G G A T C C
BclI  (2153)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsiWI  (2166)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
KasI  (2322)
1 site
G G C G C C C C G C G G
NarI  (2323)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (2324)
1 site
G G C G C C C C G C G G
PluTI  (2326)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
AarI  (2626)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BsaBI  (2638)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PspOMI  (2759)
1 site
G G G C C C C C C G G G
ApaI  (2763)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PstI  (2878)
1 site
C T G C A G G A C G T C
VP1.5 (forward primer)
18-mer  /  44% GC
1 binding site
839 .. 856  =  18 annealed bases
Tm  =  51°C
XL39 (reverse primer)
20-mer  /  55% GC
1 binding site
1200 .. 1219  =  20 annealed bases
Tm  =  59°C
AmpR
4383 .. 5243  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   4383 .. 5174  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4383 .. 5243  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   5175 .. 5243  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4383 .. 5243  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
TurboGFP
2175 .. 2873  =  699 bp
232 amino acids  =  25.7 kDa
Product: improved green fluorescent protein from
Pontellina plumata (Evdokimov et al., 2006)
mammalian codon-optimized
TurboGFP
2175 .. 2873  =  699 bp
232 amino acids  =  25.7 kDa
Product: improved green fluorescent protein from
Pontellina plumata (Evdokimov et al., 2006)
mammalian codon-optimized
hGH poly(A) signal
1137 .. 1759  =  623 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
1137 .. 1759  =  623 bp
human growth hormone polyadenylation signal
ori
3624 .. 4212  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3624 .. 4212  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
5375 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
5375 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
1788 .. 2117  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
1788 .. 2117  =  330 bp
SV40 enhancer and early promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
MCS
979 .. 1110  =  132 bp
multiple cloning site
MCS
979 .. 1110  =  132 bp
multiple cloning site
SV40 poly(A) signal
3052 .. 3173  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3052 .. 3173  =  122 bp
SV40 polyadenylation signal
AmpR promoter
5244 .. 5348  =  105 bp
AmpR promoter
5244 .. 5348  =  105 bp
T7 promoter
952 .. 970  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
952 .. 970  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
1968 .. 2103  =  136 bp
SV40 origin of replication
SV40 ori
1968 .. 2103  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter