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pCMV6-A-GFP

PrecisionShuttle™ mammalian expression vector for coexpressing an untagged ORF with a TurboGFP marker.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCMV6-A-GFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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DraIII (5608) ScaI (4936) AlwNI (3979) PciI (3563) BstZ17I (3184) BsmI (3132) AanI (3071) PfoI (2987) BstBI (2894) AanI (5733) BsrGI (301) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI - BssHII (1030) HindIII (1044) RsrII (1061) MluI (1067) NotI (1077) PaeR7I - PspXI - XhoI (1085) PmeI (1094) SacII (1108) AleI (1194) XL39 (reverse primer) (1200 .. 1219) BbsI (1317) BbvCI - Bpu10I (1420) BsmBI (1484) BstXI (1571) AgeI (1682) XcmI (1777) SexAI * (1869) SfiI (2055) StuI (2101) AvrII (2102) BclI * (2153) BsiWI (2166) KasI (2322) NarI (2323) SfoI (2324) PluTI (2326) AarI (2626) BsaBI * (2638) PspOMI (2759) ApaI (2763) PstI (2878) pCMV6-A-GFP 5805 bp
DraIII  (5608)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
ScaI  (4936)
1 site		 A G T A C T T C A T G A
AlwNI  (3979)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PciI  (3563)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BstZ17I  (3184)
1 site		 G T A T A C C A T A T G
BsmI  (3132)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
AanI  (3071)
2 sites		 T T A T A A A A T A T T
PfoI  (2987)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
BstBI  (2894)
1 site		 T T C G A A A A G C T T
AanI  (5733)
2 sites		 T T A T A A A A T A T T
BsrGI  (301)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
NdeI  (592)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site		 T A C G T A A T G C A T
Eco53kI  (924)
1 site		 G A G C T C C T C G A G
SacI  (926)
1 site		 G A G C T C C T C G A G
EcoRI  (979)
1 site