pCS6-Empty

Empty cloning vector for transient mammalian cell expression of a cDNA. Related to pCMV SPORT6.

Sequence Author: transOMIC

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AanI (3958) AanI (239) AanI (690) MluI (711) HindIII (717) PspOMI (723) ApaI (727) AbsI - PaeR7I - PspXI - XhoI (729) XbaI (741) EcoRV (749) TspMI - XmaI (752) SmaI (754) EcoRI (757) RsrII (764) AgeI (766) Acc65I (769) KpnI (773) SP6 promoter pCS6-Empty 4318 bp
AanI  (3958)
3 sites
T T A T A A A A T A T T
AanI  (239)
3 sites
T T A T A A A A T A T T
AanI  (690)
3 sites
T T A T A A A A T A T T
MluI  (711)
1 site
A C G C G T T G C G C A
HindIII  (717)
1 site
A A G C T T T T C G A A
PspOMI  (723)
1 site
G G G C C C C C C G G G
ApaI  (727)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AbsI  (729)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (729)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (729)
1 site
V C T C G A G B B G A G C T C V
XhoI  (729)
1 site
C T C G A G G A G C T C
XbaI  (741)
1 site
T C T A G A A G A T C T
EcoRV  (749)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
TspMI  (752)
1 site
C C C G G G G G G C C C
XmaI  (752)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (754)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (757)
1 site
G A A T T C C T T A A G
RsrII  (764)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AgeI  (766)
1 site
A C C G G T T G G C C A
Acc65I  (769)
1 site
G G T A C C C C A T G G
KpnI  (773)
1 site
G G T A C C C C A T G G
AmpR
2714 .. 3574  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2714 .. 3505  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2714 .. 3574  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3506 .. 3574  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2714 .. 3574  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1913 .. 2501  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1913 .. 2501  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3862 .. 4317  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3862 .. 4317  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
1150 .. 1453  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1150 .. 1453  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
946 .. 1149  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
946 .. 1149  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
125 .. 259  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
125 .. 259  =  135 bp
SV40 polyadenylation signal
MCS
711 .. 774  =  64 bp
multiple cloning site
MCS
711 .. 774  =  64 bp
multiple cloning site
loxP
2579 .. 2612  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
2579 .. 2612  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
SP6 promoter
775 .. 793  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
775 .. 793  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
M13 fwd
664 .. 680  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
664 .. 680  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
804 .. 820  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
804 .. 820  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  3560 .. 3799  =  240 bp
ORF:  79 amino acids  =  9.0 kDa
ORF:  801 .. 1067  =  267 bp
ORF:  88 amino acids  =  9.5 kDa
ORF:  2844 .. 3110  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1256 .. 1513  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  2714 .. 3574  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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