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pEF6/V5-His C

Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pEF6/V5‑His A or pEF6/V5‑His B.

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pEF6 V5-His C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SspI (3) ScaI (5482) FspI (5224) AhdI (5002) BspQI - SapI (4004) BstZ17I (3741) BsmI (3689) PfoI (3544) BlpI (3448) BsaBI * (3351) PpuMI (3212) MscI (3056) SmaI (2947) TspMI - XmaI (2945) ZraI (119) SgrDI (120) AatII (121) AflII (852) BfuAI - BspMI (997) PspOMI (1086) ApaI (1090) FseI (1177) PasI (1221) BbvCI (1375) KasI (1411) NarI (1412) SfoI (1413) PluTI (1415) Acc65I (1713) KpnI (1717) BamHI (1725) SpeI (1731) EcoRI (1748) EcoRV (1760) NotI (1775) PspXI (1781) BstEII (1786) BstBI (1796) PmeI (1876) SexAI * (2691) AvrII (2924) pEF6/V5-His C 5803 bp
SspI  (3)
1 site
A A T A T T T T A T A A
ScaI  (5482)
1 site
A G T A C T T C A T G A
FspI  (5224)
1 site
T G C G C A A C G C G T
AhdI  (5002)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (4004)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4004)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (3741)
1 site
G T A T A C C A T A T G
BsmI  (3689)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
PfoI  (3544)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BlpI  (3448)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsaBI  (3351)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PpuMI  (3212)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
MscI  (3056)
1 site
T G G C C A A C C