pF4K CMV

Flexi® vector with a kanamycin resistance marker, for protein expression in mammalian cells.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
ScaI (4078) MluI (3994) BspEI (3781) BspHI (3737) ApaLI (3331) PciI (3017) AgeI (2949) BstBI (2922) RsrII (2756) NaeI (2742) NgoMIV (2740) PflFI - Tth111I (2358) FspI (2342) BclI * (2081) BsrGI (96) SpeI (152) CMV enhancer NdeI (387) SnaBI (493) HindIII (748) BsaI (882) BbsI (928) NheI (1052) BmtI (1056) AsiSI - PvuI - SgfI (1060) BpmI (1261) DraI - PmeI (1427) Eco53kI (1440) SacI (1442) Acc65I (1444) AvaI - BmeT110I - BsoBI - KpnI - TspMI - XmaI (1448) SmaI (1450) XbaI (1459) SalI (1465) AccI (1466) SbfI (1475) BlpI (1540) NotI (1570) BtsαI (1639) PsiI (1693) HpaI (1713) MfeI (1722) XcmI (2056) pF4K CMV 4118 bp
ScaI  (4078)
1 site
A G T A C T T C A T G A
MluI  (3994)
1 site
A C G C G T T G C G C A
BspEI  (3781)
1 site
T C C G G A A G G C C T
BspHI  (3737)
1 site
T C A T G A A G T A C T
ApaLI  (3331)
1 site
G T G C A C C A C G T G
PciI  (3017)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (2949)
1 site
A C C G G T T G G C C A
BstBI  (2922)
1 site
T T C G A A A A G C T T
RsrII  (2756)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NaeI  (2742)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2740)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
PflFI  (2358)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2358)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2342)
1 site
T G C G C A A C G C G T
BclI  (2081)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsrGI  (96)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (152)
1 site
A C T A G T T G A T C A
NdeI  (387)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (493)
1 site
T A C G T A A T G C A T
HindIII  (748)
1 site
A A G C T T T T C G A A
BsaI  (882)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BbsI  (928)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
NheI  (1052)
1 site
G C T A G C C G A T C G
BmtI  (1056)
1 site
G C T A G C C G A T C G
AsiSI  (1060)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1060)
1 site
C G A T C G G C T A G C
SgfI  (1060)
1 site
G C G A T C G C C G C T A G C G
BpmI  (1261)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
DraI  (1427)
1 site
T T T A A A A A A T T T
PmeI  (1427)
1 site
G T T T A A A C C A A A T T T G
Eco53kI  (1440)
1 site
G A G C T C C T C G A G
SacI  (1442)
1 site
G A G C T C C T C G A G
Acc65I  (1444)
1 site
G G T A C C C C A T G G
AvaI  (1448)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (1448)
1 site
C Y C G R G G R G C Y C
BsoBI  (1448)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
KpnI  (1448)
1 site
G G T A C C C C A T G G
TspMI  (1448)
1 site
C C C G G G G G G C C C
XmaI  (1448)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1450)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
XbaI  (1459)
1 site
T C T A G A A G A T C T
SalI  (1465)
1 site
G T C G A C C A G C T G
AccI  (1466)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SbfI  (1475)
1 site
C C T G C A G G G G A C G T C C
BlpI  (1540)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NotI  (1570)
1 site
G C G G C C G C C G C C G G C G
BtsαI  (1639)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
PsiI  (1693)
1 site
T T A T A A A A T A T T
HpaI  (1713)
1 site
G T T A A C C A A T T G
MfeI  (1722)
1 site
C A A T T G G T T A A C
XcmI  (2056)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
NeoR/KanR
2112 .. 2906  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
2112 .. 2906  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
3078 .. 3666  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3078 .. 3666  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
138 .. 517  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
138 .. 517  =  380 bp
human cytomegalovirus immediate early enhancer
barnase
1087 .. 1422  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
1087 .. 1422  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
cer region
3782 .. 4065  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
3782 .. 4065  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
SV40 poly(A) signal
1592 .. 1713  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1592 .. 1713  =  122 bp
SV40 polyadenylation signal
T7 promoter
1033 .. 1051  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1033 .. 1051  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  1087 .. 1422  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  2284 .. 2670  =  387 bp
ORF:  128 amino acids  =  14.7 kDa
ORF:  2112 .. 2906  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2421 .. 2675  =  255 bp
ORF:  84 amino acids  =  9.8 kDa
ORF:  821 .. 1066  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
Click here to try SnapGene

Download pF4K CMV.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.