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pF5K CMV-neo

Flexi® vector with a kanamycin/G418 resistance marker, for protein expression in mammalian cells.

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pF5K CMV-neo.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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ScaI (4554) MluI (4470) BspEI (4257) ApaLI (3807) PciI (3493) BspDI - ClaI (3318) BstXI (3258) BstBI (3219) RsrII (3053) NaeI (3039) NgoMIV (3037) BssHII (2934) PflFI - Tth111I (2655) PluTI (2540) SfoI (2538) NarI (2537) KasI (2536) PacI (2324) AvrII (2300) StuI (2299) BglII (4589) BsrGI (96) SpeI (152) NdeI (387) SnaBI (493) BsaI (882) BbsI (928) NheI (1052) BmtI (1056) AsiSI - PvuI - SgfI (1060) DraI - PmeI (1427) EcoRI (1432) XbaI (1459) SalI (1465) AccI (1466) SbfI (1475) BlpI (1540) NotI (1570) Bts α I (1639) PsiI (1693) HpaI (1713) MfeI (1722) SexAI * (2067) SfiI (2253) BseRI (2296) pF5K CMV-neo 4594 bp
ScaI  (4554)
1 site
A G T A C T T C A T G A
MluI  (4470)
1 site
A C G C G T T G C G C A
BspEI  (4257)
1 site
T C C G G A A G G C C T
ApaLI  (3807)
1 site
G T G C A C C A C G T G
PciI  (3493)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspDI  (3318)
1 site
A T C G A T T A G C T A
ClaI  (3318)
1 site
A T C G A T T A G C T A
BstXI  (3258)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BstBI  (3219)
1 site
T T C G A A A A G C T T
RsrII  (3053)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NaeI  (3039)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3037)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BssHII  (2934)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PflFI  (2655)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2655)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (2540)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2538)
1 site
G G C G C C C C G C G G
NarI  (2537)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2536)
1 site
G G C G C C C C G C G G
PacI  (2324)
1 site
T T A A T T A A A A T T A A T T
AvrII  (2300)
1 site
C C T A G G G G A T C C
StuI  (2299)
1 site
A G G C C T T C C G G A
BglII  (4589)
1 site
A G A T C T T C T A G A
BsrGI  (96)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (152)
1 site
A C T A G T T G A T C A
NdeI  (387)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (493)
1 site
T A C G T A A T G C A T
BsaI  (882)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BbsI  (928)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
NheI  (1052)
1 site
G C T A G C C G A T C G
BmtI  (1056)
1 site
G C T A G C C G A T C G
AsiSI  (1060)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1060)
1 site
C G A T C G G C T A G C
SgfI  (1060)
1 site
G C G A T C G C C G C T A G C G
DraI  (1427)
1 site
T T T A A A A A A T T T
PmeI  (1427)
1 site
G T T T A A A C C A A A T T T G
EcoRI  (1432)
1 site
G A A T T C C T T A A G
XbaI  (1459)
1 site
T C T A G A A G A T C T
SalI  (1465)
1 site