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pFN23A HaloTag® CMVd2

Flexi® vector with an ampicillin resistance marker, for mammalian expression from a moderately compromised CMV promoter of a protein with an N-terminal HaloTag®.

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pFN23A HaloTag CMVd2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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BsaBI * (4134) MluI (4016) BspEI (3803) PciI (3039) AgeI (2971) BstBI (2944) AhdI (2869) AseI (2694) FspI (2646) XmnI (2269) SspI (2064) BglII (4135) HindIII (79) PflMI (184) AleI (242) BstEII (260) BseRI (262) PflFI - Tth111I (392) BmgBI (394) PasI (424) StuI * (593) BclI * (624) BspDI * - ClaI * (630) PshAI (689) SgrAI (776) BbsI (975) BssHII (1001) PaeR7I - XhoI (1019) AsiSI - PvuI - SgfI (1077) BtgI - NcoI (1080) DraI - PmeI (1444) Acc65I (1461) KpnI (1465) XbaI (1476) PstI - SbfI (1492) BfuAI - BspMI (1495) BlpI (1557) NotI (1587) AanI - PsiI (1710) HpaI (1730) MfeI (1739) SnaBI (1931) pFN23A HaloTag® CMVd2 4140 bp
BsaBI  (4134)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MluI  (4016)
1 site
A C G C G T T G C G C A
BspEI  (3803)
1 site
T C C G G A A G G C C T
PciI  (3039)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (2971)
1 site
A C C G G T T G G C C A
BstBI  (2944)
1 site
T T C G A A A A G C T T
AhdI  (2869)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AseI  (2694)
1 site
A T T A A T T A A T T A
FspI  (2646)
1 site
T G C G C A A C G C G T
XmnI  (2269)
1 site
G A A N N N N T T C C T T N N N N A A G
SspI  (2064)
1 site
A A T A T T T T A T A A
BglII  (4135)
1 site
A G A T C T T C T A G A
HindIII  (79)
1 site
A A G C T T T T C G A A
PflMI  (184)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (242)
1 site
C A C N N N N G T G G T G N N N N C A C
BstEII  (260)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BseRI  (262)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PflFI  (392)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (392)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmgBI  (394)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PasI  (424)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
StuI  (593)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
BclI  (624)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspDI  (630)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (630)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
PshAI  (689)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SgrAI  (776)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BbsI  (975)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BssHII  (1001)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PaeR7I  (1019)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1019)
1 site
C T C G A G G A G C T C
AsiSI  (1077)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1077)
1 site
C G A T C G G C T A G C
SgfI  (1077)
1 site
G C G A T C G C C G C T A G C G
BtgI  (1080)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (1080)
1 site
C C A T G