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pFN28A HaloTag® CMV-neo

Flexi® vector with ampicillin and G418 resistance markers, for mammalian expression of a protein with a cleavable N-terminal HaloTag®.

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pFN28A HaloTag CMV-neo.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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BglII (6527) MluI (6408) BspEI (6195) PciI (5431) AgeI (5363) AhdI (5261) XmnI (4661) BstXI (4156) RsrII (3951) NaeI (3937) NgoMIV (3935) PluTI (3438) SfoI (3436) NarI (3435) KasI (3434) BsrGI (96) SpeI (152) NdeI (387) NheI (1052) BmtI (1056) PflMI (1108) AleI (1166) BstEII (1184) BmgBI (1318) PasI (1348) BclI * (1548) PshAI (1613) SgrAI (1700) PaeR7I - XhoI (1943) AsiSI - PvuI - SgfI (2001) DraI - PmeI (2368) EcoRI (2373) XbaI (2400) SbfI (2416) BlpI (2481) NotI (2511) AanI - PsiI (2634) HpaI (2654) MfeI (2663) SexAI * (3008) SfiI (3194) AvrII (3241) pFN28A HaloTag® CMV-neo 6532 bp
BglII  (6527)
1 site
A G A T C T T C T A G A
MluI  (6408)
1 site
A C G C G T T G C G C A
BspEI  (6195)
1 site
T C C G G A A G G C C T
PciI  (5431)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (5363)
1 site
A C C G G T T G G C C A
AhdI  (5261)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
XmnI  (4661)
1 site
G A A N N N N T T C C T T N N N N A A G
BstXI  (4156)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
RsrII  (3951)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NaeI  (3937)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3935)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
PluTI  (3438)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3436)
1 site
G G C G C C C C G C G G
NarI  (3435)
1 site
G G C G C C C C