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pHTN HaloTag® CMV-neo

Mammalian expression vector with a traditional MCS and ampicillin and G418 resistance markers, encoding a cleavable N-terminal HaloTag®.

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pHTN HaloTag CMV-neo.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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BglII (6138) MluI (6019) BspEI (5806) PciI (5042) AgeI (4974) AhdI (4872) XmnI (4272) BamHI (3840) BstXI (3767) RsrII (3562) NaeI (3548) NgoMIV (3546) PluTI (3049) BsrGI (96) SpeI (152) NdeI (387) NheI (1052) BmtI (1056) PflMI (1108) AleI (1166) BstEII (1184) BmgBI (1318) PasI (1348) BclI * (1548) PshAI (1613) SgrAI (1700) TspMI - XmaI (1870) SmaI (1872) SalI (1936) AccI (1937) PaeR7I - XhoI (1943) AsiSI - PvuI (2001) EcoRI (2009) Eco53kI (2018) SacI (2020) SacII (2027) EcoRV (2031) XbaI (2033) PspOMI (2042) ApaI (2046) SbfI (2057) NotI (2066) BlpI (2097) PsiI (2245) HpaI (2265) MfeI (2274) Acc65I (2502) KpnI (2506) SexAI * (2619) SfiI (2805) AvrII (2852) KasI (3045) NarI (3046) SfoI (3047) pHTN HaloTag® CMV-neo 6143 bp
BglII  (6138)
1 site
A G A T C T T C T A G A
MluI  (6019)
1 site
A C G C G T T G C G C A
BspEI  (5806)
1 site
T C C G G A A G G C C T
PciI  (5042)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (4974)
1 site
A C C G G T T G G C C A
AhdI  (4872)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
XmnI  (4272)
1 site
G A A N N N N T T C C T T N N N N A A G
BamHI  (3840)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BstXI  (3767)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
RsrII  (3562)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NaeI  (3548)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3546)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
PluTI  (3049)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BsrGI  (96)
1 site
T G T A C A A C