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pHom-Mem1

Mammalian vector encoding FKBP homodimerizer domains, for creating membrane-associated fusion proteins that can be dimerized with a drug. Also known as pC4M-FV2E.

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pHom-Mem1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PvuII (5614) NaeI (5412) NgoMIV (5410) DraIII (5309) XmnI (4460) BsaI (3910) AhdI (3849) BspQI - SapI (2840) MluI (1) SnaBI (298) EcoRI (656) AfeI (707) XbaI (710) BlpI (930) AarI (1039) TspMI - XmaI (1158) SmaI (1160) SpeI (1364) BsiWI (1374) BamHI (1409) KflI (1438) HincII (1590) MfeI (1780) PspOMI (1955) ApaI (1959) MscI (2100) BstXI (2108) BglII (2129) PflMI (2335) AbsI - PspXI (2578) AvrII (2601) StuI (2606) SfiI (2655) pHom-Mem1 5726 bp
PvuII  (5614)
1 site
C A G C T G G T C G A C
NaeI  (5412)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (5410)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (5309)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (4460)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaI  (3910)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3849)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (2840)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2840)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
MluI  (1)
1 site
A C G C G T T G C G C A
SnaBI  (298)
1 site
T A C G T A A T G C A T
EcoRI  (656)
1 site
G A A T T C C T T A A G
AfeI  (707)
1 site
A G C G C T T C G C G A
XbaI  (710)
1 site
T C T A G A A G A T C T
BlpI  (930)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AarI  (1039)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
TspMI  (1158)
1 site
C C C G G G G G G C C C
XmaI  (1158)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1160)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SpeI  (1364)
1 site
A C T A G T T G A T C A
BsiWI  (1374)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BamHI  (1409)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KflI  (1438)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
HincII  (1590)
1 site
G T Y R A C C A R Y T G
MfeI  (1780)
1 site
C A A T T G G T T A A C
PspOMI  (1955)
1 site
G G G C C C C C C G G G
ApaI  (1959)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
MscI  (2100)
1 site
T G G C C A A C C G G T
BstXI  (2108)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BglII  (2129)
1 site
A G A T C T T C T A G A
PflMI  (2335)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AbsI  (2578)
1 site
C C T C G A G G G G A G C T C C
PspXI  (2578)
1 site
V C T C G A G B B G A G C T C V
AvrII  (2601)
1 site
C C T A G G G G A T C C
StuI  (2606)
1 site
A G G C C T T C C G G A
SfiI  (2655)
1 site