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Plasmid Files

pPTuner IRES2

Vector for fusing a destabilization domain to the N-terminus of a partner protein, with co-expression of AcGFP1.

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pPTuner IRES2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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BsaI (4650) RsrII (4177) PflFI - Tth111I (3779) FspI (3763) BspDI * - ClaI * (3501) StuI (3482) SfiI (3436) SexAI * (3250) AseI (7) NdeI (234) SnaBI (340) BsmBI (622) AfeI (926) BglII (939) PaeR7I - XhoI (943) Eco53kI (948) SacI (950) EcoRI (959) PstI (968) SalI (969) AccI (970) SacII (982) BamHI (990) AclI (1020) XmnI (1209) PmlI (1313) BmgBI (1540) BstXI (1583) AleI (1764) BstEII (1766) Bpu10I (1776) BssHII (1910) EcoNI (2208) PpuMI (2213) NotI (2305) XbaI * (2315) MfeI (2411) HpaI (2424) AflII (2543) pPTuner IRES2 5637 bp
BsaI  (4650)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (4177)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (3779)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3779)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (3763)
1 site
T G C G C A A C G C G T
BspDI  (3501)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (3501)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (3482)
1 site
A G G C C T T C C G G A
SfiI  (3436)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SexAI  (3250)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
BsmBI  (622)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
AfeI  (926)
1 site
A G C G C T T C G C G A
BglII  (939)
1 site
A G A T C T T C T A G A
PaeR7I  (943)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (943)
1 site
C T C G A G G A G C T C
Eco53kI  (948)
1 site
G A G C T C C T C G A G
SacI  (950)
1 site
G A G C T C C T C G A G
EcoRI  (959)
1 site
G A A T T C C T T A A G
PstI  (968)
1 site
C T G C A G G A C G T C
SalI  (969)
1 site
G T C G A C C A G C T G
AccI  (970)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SacII  (982)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BamHI  (990)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AclI  (1020)
1 site
A A C G T T T T G C A A
XmnI  (1209)
1 site
G A A N N N N T T C C T T N N N N A A G
PmlI  (1313)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BmgBI  (1540)
1 site