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Plasmid Files

pSecTag C

Mammalian expression vector for the secretion of C-terminally 6xHis-tagged proteins (replaced by pSecTag2 C). For other reading frames, use pSecTag A or pSecTag B.

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pSecTag C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (5172) SspI (5055) ScaI (4731) PvuI (4621) FspI (4473) AhdI (4251) PciI (3358) BspQI - SapI (3242) BstZ17I (2979) BsmI (2927) FseI (2619) BglII (12) MfeI (161) Bpu10I (180) NruI (208) MluI (228) NdeI (484) NheI (895) BmtI (899) SfiI (977) HindIII (1005) Acc65I (1011) KpnI (1015) BamHI (1023) EcoRI (1046) PstI (1055) EcoRV (1058) NotI (1073) PaeR7I - PspXI - XhoI (1079) XbaI (1085) EcoO109I - PspOMI (1091) ApaI (1095) PmeI (1166) BbsI (1377) StuI (2213) AvrII (2214) MscI (2346) SgrAI (2457) BmgBI (2477) pSecTag C 5174 bp
SgrDI  (5172)
1 site
C G T C G A C G G C A G C T G C
SspI  (5055)
1 site
A A T A T T T T A T A A
ScaI  (4731)
1 site
A G T A C T T C A T G A
PvuI  (4621)
1 site
C G A T C G G C T A G C
FspI  (4473)
1 site
T G C G C A A C G C G T
AhdI  (4251)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (3358)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3242)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3242)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (2979)
1 site
G T A T A C C A T A T G
BsmI  (2927)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
FseI  (2619)
1 site