Resources
Plasmid Files

pTCN

Mammalian cell vector with a neomycin resistance marker, for expressing a cDNA from the CMV promoter.

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pTCN Sequence and MappTCN.dna
Map and Sequence File   
Sequence Author:  transOMIC
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 BamHI (584) EcoRV (615) HindIII (644) NotI (700) XbaI (721) pTCN 5193 bp
BamHI  (584)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (615)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
HindIII  (644)
1 site
A A G C T T T T C G A A
NotI  (700)
1 site
G C G G C C G C C G C C G G C G
XbaI  (721)
1 site
T C T A G A A G A T C T
AmpR
3947 .. 4807  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3947 .. 4738  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3947 .. 4807  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4739 .. 4807  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3947 .. 4807  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
NeoR/KanR
1651 .. 2445  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
1651 .. 2445  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
ori
3191 .. 3776  =  586 bp
high-copy-number colE1/pMB1/pBR322/pUC origin
of replication
ori
3191 .. 3776  =  586 bp
high-copy-number colE1/pMB1/pBR322/pUC origin
of replication
f1 ori
813 .. 1241  =  429 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
813 .. 1241  =  429 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
5178 .. 364  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
5178 .. 364  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
1255 .. 1584  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
1255 .. 1584  =  330 bp
SV40 enhancer and early promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
2619 .. 2740  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2619 .. 2740  =  122 bp
SV40 polyadenylation signal
AmpR promoter
4808 .. 4912  =  105 bp
AmpR promoter
4808 .. 4912  =  105 bp
SV40 ori
1435 .. 1570  =  136 bp
SV40 origin of replication
SV40 ori
1435 .. 1570  =  136 bp
SV40 origin of replication
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