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pTRE-Dual2

Vector for co-expressing mCherry and another gene with the Tet-On® Advanced or Tet-Off® Advanced system.

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pTRE-Dual2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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ZraI (3813) SspI (3697) EarI (3688) ScaI (3373) PvuI (3263) FspI (3115) AseI (3065) BsaI (2954) AatII (3815) Eco53kI (292) SacI (294) EcoRI (323) PstI - SbfI (690) BbvCI - Bpu10I (873) PvuII (913) BsgI (929) SgrAI (1016) XcmI (1021) BsrGI (1035) NheI (1046) BmtI (1050) PspOMI (1166) ApaI (1170) AvrII (1204) HindIII (1277) PmlI (1369) AarI - BfuAI - BspMI (1392) DraIII (1416) Acc65I (1494) KpnI (1498) BmgBI (1596) EagI - NotI - SacII (1641) BglII (1649) BamHI (1655) BspDI - ClaI (1662) SalI (1667) AccI (1668) EcoRV (1675) NdeI (1680) XbaI (1685) MfeI (1784) HpaI (1797) PsiI (1817) pTRE-Dual2 3884 bp
ZraI  (3813)
1 site
G A C G T C C T G C A G
SspI  (3697)
1 site
A A T A T T T T A T A A
EarI  (3688)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI recognition sequence.
Sticky ends from different EarI sites may not be compatible.
ScaI  (3373)
1 site
A G T A C T T C A T G A
PvuI  (3263)
1 site
C G A T C G G C T A G C
FspI  (3115)
1 site
T G C G C A A C G C G T
AseI  (3065)
1 site
A T T A A T T A A T T A
BsaI  (2954)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AatII  (3815)
1 site
G A C G T C C T G C A G
Eco53kI  (292)
1 site
G A G C T C C T C G A G
SacI  (294)
1 site
G A G C T C C T C G A G
EcoRI  (323)
1 site
G A A T T C C T T A A G
PstI  (690)
1 site
C T G C A G G A C G T C
SbfI  (690)
1 site
C C T G C A G G G G A C G T C C
BbvCI  (873)
1 site
C C T C A G C G G A G T C G
Bpu10I  (873)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (913)
1 site
C A G C T G G T C G A C
BsgI  (929)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SgrAI  (1016)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XcmI  (1021)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrGI  (1035)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NheI  (1046)
1 site
G C T A G C C G A T C G
BmtI  (1050)
1 site
G C T A G C C G A T C G
PspOMI  (1166)
1 site
G G G C C C C C C G G G
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