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pTRE-Tight-BI-DsRed-Express

Vector for co-expressing DsRed-Express and another gene with the Tet-On® Advanced or Tet-Off® Advanced system.

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pTRE-Tight-BI-DsRed-Express.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (3412) FspAI (3362) PstI - SbfI (3074) BbsI (2986) EcoO109I (2919) PflMI * (2895) SexAI * (2888) MscI (2864) BsgI (2833) AleI (2783) BssHII (2762) XbaI (2728) SspI (2418) EarI (2409) XmnI (2213) ScaI (2094) TatI (2092) PvuI (1984) AseI (1786) BglI (1734) PaeR7I - PspXI - XhoI (1) Acc65I (335) KpnI - TspMI - XmaI (339) SmaI (341) BamHI (344) MluI (362) NheI (368) BmtI (372) EagI - NotI (375) BspDI - ClaI (383) HindIII (388) SalI (394) AccI (395) EcoRV (402) PciI (721) NspI (725) DrdI (829) AlwNI (1137) BsaI (1675) BsrFI (1694) pTRE-Tight-BI-DsRed-Express 3491 bp
EcoRI  (3412)
1 site
G A A T T C C T T A A G
FspAI  (3362)
1 site
R T G C G C A Y Y A C G C G T R
PstI  (3074)
1 site
C T G C A G G A C G T C
SbfI  (3074)
1 site
C C T G C A G G G G A C G T C C
BbsI  (2986)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (2919)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PflMI  (2895)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
SexAI  (2888)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MscI  (2864)
1 site
T G G C C A A C C G G T
BsgI  (2833)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (2783)
1 site
C A C N N N N G T G G T G N N N N C A C
BssHII  (2762)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
XbaI  (2728)
1 site
T C T A G A A G A T C T
SspI  (2418)
1 site
A A T A T T T T A T A A
EarI  (2409)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI recognition sequence.
Sticky ends from different EarI sites may not be compatible.
XmnI  (2213)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2094)
1 site
A G T A C T T C A T G A
TatI  (2092)
1 site
W G T A C W W C A T G W
PvuI  (1984)
1 site
C G A T C G G C T A G C
AseI  (1786)
1 site
A