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Plasmid Files

pTRE3G-BI-ZsGreen1

Vector for doxycycline-inducible expression of a gene together with the ZsGreen1 fluorescent protein.

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pTRE3G-BI-ZsGreen1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (3545) NcoI (3538) BclI * (3454) PflFI - Tth111I (3305) BseRI (3182) BmgBI (3049) BfuAI - BspMI (3025) BsrGI (2986) BtgZI (2883) PstI (2844) PshAI (2842) XbaI (2834) AatII (2642) ZraI (2640) SspI (2524) XmnI (2319) ScaI (2200) PvuI (2090) FspI (1942) AseI (1892) StuI (40) PaeR7I - PspXI - XhoI (70) HindIII (322) EcoO109I - PspOMI (462) ApaI (466) BglII (468) BamHI (480) BspDI * - ClaI * (487) EagI - NotI (495) MreI (500) EcoRV (508) AflIII - PciI (827) DrdI (935) BseYI (1131) PspFI (1135) AlwNI (1243) BsaI (1781) pTRE3G-BI-ZsGreen1 3555 bp
EcoRI  (3545)
1 site
G A A T T C C T T A A G
NcoI  (3538)
1 site
C C A T G G G G T A C C
BclI  (3454)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PflFI  (3305)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3305)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BseRI  (3182)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BmgBI  (3049)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BfuAI  (3025)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3025)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsrGI  (2986)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BtgZI  (2883)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
PstI  (2844)
1 site
C T G C A G G A C G T C
PshAI  (2842)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
XbaI  (2834)
1 site
T C T A G A A G A T C T
AatII  (2642)
1 site
G A C G T C C T G C A G
ZraI  (2640)
1 site
G A C G T C C T G C A G
SspI  (2524)
1 site
A A T A T T T T A T A A
XmnI  (2319)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2200)
1 site
A G T A C T T C A T G A
PvuI  (2090)
1 site
C G A T C G G C T A G C
FspI  (1942)
1 site
T G C G C A A C G C G T
AseI  (1892)
1 site
A T T A A T T A A T T A
StuI  (40)
1 site
A G G C C T T C C G G A
PaeR7I  (70)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (70)
1 site
V C T C G A G B B G A G C T C V
XhoI  (70)
1 site
C T C G A G G A G C T C
HindIII  (322)
1 site
A A G C T T T T C G A A
EcoO109I  (462)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (462)
1 site
G G G C C C C C C G G G
ApaI  (466)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BglII  (468)
1 site
A G A T C T T C T A G A
BamHI  (480)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BspDI  (487)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (487)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
EagI  (495)
1 site
C G G C C G G C C G