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Plasmid Files

pTRE3G-BI-mCherry

Vector for doxycycline-inducible expression of a gene together with the mCherry fluorescent protein.

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pTRE3G-BI-mCherry.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (3554) MscI (3519) BsaAI (3319) PstI - SbfI (3198) PflMI (3113) BbsI (3110) BbvCI - Bpu10I (3008) PvuII (2971) BsgI (2957) XcmI (2864) BsrGI (2845) XbaI (2834) AatII (2642) ZraI (2640) SspI (2524) XmnI (2319) ScaI (2200) PvuI (2090) FspI (1942) AseI (1892) BglI (1840) BsaI (1781) PaeR7I - PspXI - XhoI (70) HindIII (322) SalI (452) AccI (453) EcoO109I - PspOMI (462) ApaI (466) BglII (468) BamHI (480) BspDI * - ClaI * (487) EagI - NotI (495) MreI - NgoMIV (500) NaeI (502) EcoRV (508) AflIII - PciI (827) NspI (831) BmrI (1760) pTRE3G-BI-mCherry 3564 bp
EcoRI  (3554)
1 site
G A A T T C C T T A A G
MscI  (3519)
1 site
T G G C C A A C C G G T
BsaAI  (3319)
1 site
Y A C G T R R T G C A Y
PstI  (3198)
1 site
C T G C A G G A C G T C
SbfI  (3198)
1 site
C C T G C A G G G G A C G T C C
PflMI  (3113)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BbsI  (3110)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbvCI  (3008)
1 site
C C T C A G C G G A G T C G
Bpu10I  (3008)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (2971)
1 site
C A G C T G G T C G A C
BsgI  (2957)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XcmI  (2864)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrGI  (2845)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
XbaI  (2834)
1 site
T C T A G A A G A T C T
AatII  (2642)
1 site
G A C G T C C T G C A G
ZraI  (2640)
1 site
G A C G T C C T G C A G
SspI  (2524)
1 site
A A T A T T T T A T A A
XmnI  (2319)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2200)
1 site
A G T A C T T C A T G A
PvuI  (2090)
1 site
C G A T C G G C T A G C
FspI  (1942)
1 site
T G C G C A A C G C G T
AseI  (1892)
1 site
A T T A A T T A A T T A
BglI  (1840)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (1781)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PaeR7I  (70)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (70)
1 site
V C T C G A G B B G A G C T C V
XhoI  (70)
1 site
C T C G A G G A G C T C
HindIII  (322)
1 site
A A G C T T T T C G A A
SalI  (452)
1 site
G T C G A C C A G C T G
AccI  (453)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
EcoO109I  (462)
1 site