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pTRE3G-mCherry

Vector for doxycycline-inducible expression of a gene together with the mCherry fluorescent protein.

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pTRE3G-mCherry.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (4710) AatII (4639) ZraI (4637) ScaI (4197) PvuI (4087) FspI (3939) BsaI (3778) BspQI - SapI (2708) HpaI (2506) MfeI (2493) BsaBI * (2405) PaeR7I - PspXI - XhoI (1) Eco53kI (292) SacI (294) SalI (383) AccI (384) NcoI (822) BbvCI - Bpu10I (927) PvuII (967) AleI (1031) SgrAI (1070) XcmI (1075) BsrGI (1089) EagI (1100) NgoMIV (1102) NaeI (1104) FseI (1106) PspOMI (1221) ApaI (1225) AvrII (1259) PmlI (1424) AarI - BfuAI - BspMI (1447) DraIII (1471) Acc65I (1549) KpnI (1553) BmgBI (1651) EcoRV (1703) MluI (1707) NdeI (1714) NheI (1719) BmtI (1723) BamHI (1731) BtgZI (1768) pTRE3G-mCherry 4715 bp
EcoRI  (4710)
1 site
G A A T T C C T T A A G
AatII  (4639)
1 site
G A C G T C C T G C A G
ZraI  (4637)
1 site
G A C G T C C T G C A G
ScaI  (4197)
1 site
A G T A C T T C A T G A
PvuI  (4087)
1 site
C G A T C G G C T A G C
FspI  (3939)
1 site
T G C G C A A C G C G T
BsaI  (3778)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BspQI  (2708)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2708)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
HpaI  (2506)
1 site