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Plasmid Files

pTracer™-CMV2

Mammalian vector with a CMV promoter, for co-expression with GFP fused to a Zeocin™ resistance marker. See also pTracer™‑CMV.

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pTracer-CMV2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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PmeI (904) Acc65I (917) KpnI (921) BstXI (948) EcoRI (952) EcoRV (964) BstXI (974) NotI (979) XbaI (991) PmeI (1006) pTracer™-CMV2 6210 bp
PmeI  (904)
2 sites
G T T T A A A C C A A A T T T G
Acc65I  (917)
1 site
G G T A C C C C A T G G
KpnI  (921)
1 site
G G T A C C C C A T G G
BstXI  (948)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (952)
1 site
G A A T T C C T T A A G
EcoRV  (964)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstXI  (974)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (979)
1 site
G C G G C C G C C G C C G G C G
XbaI  (991)
1 site
T C T A G A A G A T C T
PmeI  (1006)
2 sites
G T T T A A A C C A A A T T T G
EF-1α promoter
1309 .. 2487  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
1309 .. 2487  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
AmpR
5214 .. 6074  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   5214 .. 6005  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5214 .. 6074  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6006 .. 6074  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5214 .. 6074  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Cycle 3 GFP
2677 .. 3375  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2677 .. 3375  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
ori
4455 .. 5043  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4455 .. 5043  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
BleoR
3376 .. 3753  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
3376 .. 3753  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
3883 .. 4004  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3883 .. 4004  =  122 bp
SV40 polyadenylation signal
bGH poly(A) signal
1028 .. 1139  =  112 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1028 .. 1139  =  112 bp
bovine growth hormone polyadenylation signal
MCS
901 .. 1010  =  110 bp
multiple cloning site
MCS
901 .. 1010  =  110 bp
multiple cloning site
AmpR promoter
6075 .. 6179  =  105 bp
AmpR promoter
6075 .. 6179  =  105 bp
EM7 promoter
2611 .. 2658  =  48 bp
synthetic bacterial promoter
EM7 promoter
2611 .. 2658  =  48 bp
synthetic bacterial promoter
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2504 .. 2522  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2504 .. 2522  =  19 bp
promoter for bacteriophage T7 RNA polymerase
EF-1α intron A
1540 .. 2478  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
1540 .. 2478  =  939 bp
intron upstream of the start codon of human EF-1α
ORF:  2677 .. 3753  =  1077 bp
ORF:  358 amino acids  =  40.0 kDa
ORF:  5344 .. 5610  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1865 .. 2302  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  1944 .. 2177  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  3276 .. 3659  =  384 bp
ORF:  127 amino acids  =  13.7 kDa
ORF:  5214 .. 6074  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1607 .. 2227  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
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