Plasmid Files |
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![]() | pZeoSV2(+).dna |
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After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BstXI sites may not be compatible. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Sticky ends from different BstXI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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ApaI can be used between 25°C and 37°C. |
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ori 873 .. 1461 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 873 .. 1461 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 1816 .. 2271 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 1816 .. 2271 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
BleoR 2998 .. 3372 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
BleoR 2998 .. 3372 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
SV40 promoter 22 .. 351 = 330 bp SV40 enhancer and early promoter |
SV40 promoter 22 .. 351 = 330 bp SV40 enhancer and early promoter |
CMV enhancer 2307 .. 2610 = 304 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 2307 .. 2610 = 304 bp human cytomegalovirus immediate early enhancer |
bGH poly(A) signal 566 .. 790 = 225 bp bovine growth hormone polyadenylation signal |
bGH poly(A) signal 566 .. 790 = 225 bp bovine growth hormone polyadenylation signal |
CMV promoter 2611 .. 2814 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 2611 .. 2814 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
SV40 poly(A) signal 3386 .. 3507 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 3386 .. 3507 = 122 bp SV40 polyadenylation signal |
MCS 433 .. 548 = 116 bp multiple cloning site |
MCS 433 .. 548 = 116 bp multiple cloning site |
EM7 promoter 2932 .. 2979 = 48 bp synthetic bacterial promoter |
EM7 promoter 2932 .. 2979 = 48 bp synthetic bacterial promoter |
T7 promoter 401 .. 419 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 401 .. 419 = 19 bp promoter for bacteriophage T7 RNA polymerase |
SV40 ori 202 .. 337 = 136 bp SV40 origin of replication |
SV40 ori 202 .. 337 = 136 bp SV40 origin of replication |
ORF: 2998 .. 3372 = 375 bp ORF: 124 amino acids = 13.8 kDa |
ORF: 764 .. 1198 = 435 bp ORF: 144 amino acids = 16.3 kDa |
ORF: 1788 .. 2033 = 246 bp ORF: 81 amino acids = 8.6 kDa |
ORF: 2991 .. 3278 = 288 bp ORF: 95 amino acids = 10.1 kDa |
ORF: 2693 .. 2953 = 261 bp ORF: 86 amino acids = 9.3 kDa |
ORF: 556 .. 903 = 348 bp ORF: 115 amino acids = 12.7 kDa |
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