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pZeoSV2(+)

Constitutive mammalian expression vector with the SV40 promoter and a Zeocin™ resistance marker. See also pZeoSV2(‑).

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pZeoSV2(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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NheI (433) BmtI (437) PmeI (442) AflII (446) HindIII (449) Acc65I (455) KpnI (459) BamHI (467) SpeI (473) BstXI (486) EcoRI (490) PstI (499) EcoRV (502) BstXI (512) NotI (517) PaeR7I - PspXI - XhoI (523) PspOMI (535) ApaI (539) PmeI (544) pZeoSV2(+) 3515 bp
NheI  (433)
1 site		 G C T A G C C G A T C G
BmtI  (437)
1 site		 G C T A G C C G A T C G
PmeI  (442)
2 sites		 G T T T A A A C C A A A T T T G
AflII  (446)
1 site		 C T T A A G G A A T T C
HindIII  (449)
1 site		 A A G C T T T T C G A A
Acc65I  (455)
1 site		 G G T A C C C C A T G G
KpnI  (459)
1 site		 G G T A C C C C A T G G
BamHI  (467)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SpeI  (473)
1 site		 A C T A G T T G A T C A
BstXI  (486)
2 sites		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
EcoRI  (490)
1 site		 G A A T T C C T T A A G
PstI  (499)
1 site		 C T G C A G G A C G T C
EcoRV  (502)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstXI  (512)
2 sites		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
NotI  (517)
1 site		 G C G G C C G C C G C C G G C G
PaeR7I  (523)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (523)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (523)
1 site		 C T C G A G G A G C T C
PspOMI  (535)
1 site		 G G G C C C C C C G G G
ApaI  (539)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PmeI  (544)
2 sites		 G T T T A A A C C A A A T T T G
ori
873 .. 1461  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
873 .. 1461  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1816 .. 2271  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1816 .. 2271  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
BleoR
2998 .. 3372  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
2998 .. 3372  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
SV40 promoter
22 .. 351  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
22 .. 351  =  330 bp
SV40 enhancer and early promoter
CMV enhancer
2307 .. 2610  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2307 .. 2610  =  304 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
566 .. 790  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
566 .. 790  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
2611 .. 2814  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2611 .. 2814  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
3386 .. 3507  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3386 .. 3507  =  122 bp
SV40 polyadenylation signal
MCS
433 .. 548  =  116 bp
multiple cloning site
MCS
433 .. 548  =  116 bp
multiple cloning site
EM7 promoter
2932 .. 2979  =  48 bp
synthetic bacterial promoter
EM7 promoter
2932 .. 2979  =  48 bp
synthetic bacterial promoter
T7 promoter
401 .. 419  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
401 .. 419  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
202 .. 337  =  136 bp
SV40 origin of replication
SV40 ori
202 .. 337  =  136 bp
SV40 origin of replication
ORF:  2998 .. 3372  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
ORF:  764 .. 1198  =  435 bp
ORF:  144 amino acids  =  16.3 kDa
ORF:  1788 .. 2033  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
ORF:  2991 .. 3278  =  288 bp
ORF:  95 amino acids  =  10.1 kDa
ORF:  2693 .. 2953  =  261 bp
ORF:  86 amino acids  =  9.3 kDa
ORF:  556 .. 903  =  348 bp
ORF:  115 amino acids  =  12.7 kDa
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