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Plasmid Files

pZeoSV2(-)

Constitutive mammalian expression vector with the SV40 promoter and a Zeocin™ resistance marker. See also pZeoSV2(+).

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pZeoSV2(-).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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NheI (433) BmtI (437) PmeI (442) PspOMI (447) ApaI (451) PaeR7I - PspXI - XhoI (459) NotI (465) BstXI (478) EcoRV (484) PstI (491) EcoRI (492) BstXI (504) SpeI (509) BamHI (515) Acc65I (527) KpnI (531) HindIII (533) AflII (536) PmeI (544) pZeoSV2(-) 3515 bp
NheI  (433)
1 site
G C T A G C C G A T C G
BmtI  (437)
1 site
G C T A G C C G A T C G
PmeI  (442)
2 sites
G T T T A A A C C A A A T T T G
PspOMI  (447)
1 site
G G G C C C C C C G G G
ApaI  (451)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PaeR7I  (459)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (459)
1 site
V C T C G A G B B G A G C T C V
XhoI  (459)
1 site
C T C G A G G A G C T C
NotI  (465)
1 site
G C G G C C G C C G C C G G C G
BstXI  (478)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRV  (484)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PstI  (491)
1 site
C T G C A G G A C G T C
EcoRI  (492)
1 site
G A A T T C C T T A A G
BstXI  (504)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SpeI  (509)
1 site
A C T A G T T G A T C A
BamHI  (515)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (527)
1 site
G G T A C C C C A T G G
KpnI  (531)
1 site
G G T A C C C C A T G G
HindIII  (533)
1 site
A A G C T T T T C G A A
AflII  (536)
1 site
C T T A A G G A A T T C
PmeI  (544)
2 sites
G T T T A A A C C A A A T T T G
ori
873 .. 1461  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
873 .. 1461  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1816 .. 2271  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1816 .. 2271  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
BleoR
2998 .. 3372  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
2998 .. 3372  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
SV40 promoter
22 .. 351  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
22 .. 351  =  330 bp
SV40 enhancer and early promoter
CMV enhancer
2307 .. 2610  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2307 .. 2610  =  304 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
566 .. 790  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
566 .. 790  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
2611 .. 2814  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2611 .. 2814  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
3386 .. 3507  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3386 .. 3507  =  122 bp
SV40 polyadenylation signal
MCS
433 .. 548  =  116 bp
multiple cloning site
MCS
433 .. 548  =  116 bp
multiple cloning site
EM7 promoter
2932 .. 2979  =  48 bp
synthetic bacterial promoter
EM7 promoter
2932 .. 2979  =  48 bp
synthetic bacterial promoter
T7 promoter
401 .. 419  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
401 .. 419  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
202 .. 337  =  136 bp
SV40 origin of replication
SV40 ori
202 .. 337  =  136 bp
SV40 origin of replication
ORF:  2998 .. 3372  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
ORF:  764 .. 1198  =  435 bp
ORF:  144 amino acids  =  16.3 kDa
ORF:  1788 .. 2033  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
ORF:  2991 .. 3278  =  288 bp
ORF:  95 amino acids  =  10.1 kDa
ORF:  2693 .. 2953  =  261 bp
ORF:  86 amino acids  =  9.3 kDa
ORF:  556 .. 903  =  348 bp
ORF:  115 amino acids  =  12.7 kDa
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