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pcDNA3.1+/N-GST(TEV)

Mammalian vector with a CMV promoter for expressing proteins with an N-terminal and TEV protease-cleavable GST tag.

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pcDNA3.1+ N-GST(TEV).dna
Map and Sequence File:    Download    Open   
Sequence Author:  GenScript
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SgrDI (6090) SspI (5973) PvuI (5539) AhdI (5169) BstZ17I (3897) BsmI (3845) PfoI (3700) RsrII (3441) BssHII (3322) PflFI - Tth111I (3043) BglII (12) MfeI (161) Bpu10I (180) NruI (208) MluI (228) SpeI (249) NdeI (484) SnaBI (590) Eco53kI (816) SacI (818) NheI (895) BmtI (899) AflII (908) HindIII (911) EcoNI (933) BsgI (1209) SwaI (1350) BamHI (1607) EcoRI (1613) EcoRV (1625) BstXI (1635) NotI (1640) PaeR7I - PspXI - XhoI (1646) XbaI (1652) PspOMI (1658) ApaI (1662) BbsI (1878) DraIII (2192) SexAI * (2482) BseRI (2711) StuI (2714) AvrII (2715) TspMI - XmaI (2736) SmaI (2738) BsaBI * (2784) KasI (2924) NarI (2925) SfoI (2926) PluTI (2928) pcDNA3.1+/N-GST(TEV) 6092 bp
SgrDI  (6090)
1 site
C G T C G A C G G C A G C T G C
SspI  (5973)
1 site
A A T A T T T T A T A A
PvuI  (5539)
1 site
C G A T C G G C T A G C
AhdI  (5169)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstZ17I  (3897)
1 site
G T A T A C C A T A T G
BsmI  (3845)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
PfoI  (3700)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3441)
1 site
C G G W C C G G C C W G G