Resources
Plasmid Files

pcDNA3.1+/N-GST(Thrombin)

Mammalian vector with a CMV promoter for expressing proteins with an N-terminal and thrombin-cleavable GST tag.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pcDNA3.1+ N-GST(Thrombin).dna
Map and Sequence File:    Download    Open   
Sequence Author:  GenScript
Download Free Trial Get SnapGene Viewer


SgrDI (6078) SspI (5961) PvuI (5527) AhdI (5157) BstZ17I (3885) BsmI (3833) PfoI (3688) RsrII (3429) BssHII (3310) PflFI - Tth111I (3031) BglII (12) MfeI (161) Bpu10I (180) NruI (208) MluI (228) SpeI (249) NdeI (484) SnaBI (590) Eco53kI (816) SacI (818) NheI (895) BmtI (899) AflII (908) HindIII (911) EcoNI (933) BsgI (1209) SwaI (1350) BamHI (1595) EcoRI (1601) EcoRV (1613) BstXI (1623) NotI (1628) PaeR7I - PspXI - XhoI (1634) XbaI (1640) PspOMI (1646) ApaI (1650) BbsI (1866) DraIII (2180) SexAI * (2470) BseRI (2699) StuI (2702) AvrII (2703) TspMI - XmaI (2724) SmaI (2726) BsaBI * (2772) KasI (2912) NarI (2913) SfoI (2914) PluTI (2916) pcDNA3.1+/N-GST(Thrombin) 6080 bp
SgrDI  (6078)
1 site
C G T C G A C G G C A G C T G C
SspI  (5961)
1 site
A A T A T T T T A T A A
PvuI  (5527)
1 site
C G A T C G G