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pcDNA3.1+/N-HA

Mammalian vector with a CMV promoter for expressing N-terminally HA-tagged proteins.

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pcDNA3.1+ N-HA.dna
Map and Sequence File:    Download    Open   
Sequence Author:  GenScript
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SgrDI (5448) SspI (5331) ScaI (5007) PvuI (4897) AhdI (4527) PciI (3634) BstZ17I (3255) BsmI (3203) PfoI (3058) BstBI (2965) RsrII (2799) BglII (12) MfeI (161) Bpu10I (180) NruI (208) MluI (228) SpeI (249) NdeI (484) SnaBI (590) NheI (895) BmtI (899) AflII (908) HindIII (911) Acc65I (953) KpnI (957) BamHI (965) EcoRI (971) EcoRV (983) BstXI (993) NotI (998) PaeR7I - PspXI - XhoI (1004) XbaI (1010) EcoO109I - PspOMI (1016) ApaI (1020) BbsI (1236) DraIII (1550) SexAI * (1840) BseRI (2069) StuI (2072) AvrII (2073) TspMI - XmaI (2094) SmaI (2096) BsaBI * (2142) KasI (2282) NarI (2283) SfoI (2284) PluTI (2286) MscI (2365) PflFI - Tth111I (2401) BssHII (2680) pcDNA3.1+/N-HA 5450 bp
SgrDI  (5448)
1 site
C G T C G A C G G C A G C T G C
SspI  (5331)
1 site
A A T A T T T T A T A A
ScaI  (5007)
1 site
A G T A C T T C A T G A
PvuI  (4897)
1 site
C G A T C G G C T A G C
AhdI  (4527)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (3634)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3255)
1 site
G T A T A C C A T A T G
BsmI  (3203)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
PfoI  (3058)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (2965)
1 site
T T C G A A A A G C T T
RsrII  (2799)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BglII  (12)
1 site
A G A T C T T C T A G A
MfeI  (161)
1 site
C A A T T G G T T A A C
Bpu10I  (180)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NruI  (208)
1 site
T C G C G A A G C G C T
MluI  (228)
1 site
A C G C G T T G C G C A
SpeI  (249)
1 site
A C T A G T T G A T C A
NdeI  (484)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (590)
1 site
T A C G T A A T G C A T
NheI  (895)
1 site
G C T A G C