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Plasmid Files

pcDNA™3.1/NT-GFP

Vector for expressing N-terminally GFP-tagged proteins in mammalian cells.

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pcDNA3.1 NT-GFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (6158) SspI (6041) ScaI (5717) PvuI (5607) AhdI (5237) PciI (4344) BsmI (3913) PfoI (3768) BssHII (3390) PflFI - Tth111I (3111) PluTI (2996) BglII (12) Bpu10I (180) NruI (208) SnaBI (590) HindIII (1028) BsrGI (1183) AgeI (1633) NheI (1639) BmtI (1643) Acc65I (1645) KpnI (1649) EcoRI (1680) EcoRV (1692) NotI (1707) XbaI (1719) EcoO109I - PspOMI (1725) ApaI (1729) PmeI (1734) BbsI (1945) DraIII (2259) SexAI * (2550) BseRI (2779) StuI (2782) AvrII (2783) TspMI - XmaI (2804) SmaI (2806) BsaBI * (2852) KasI (2992) NarI (2993) SfoI (2994) pcDNA™3.1/NT-GFP 6160 bp
SgrDI  (6158)
1 site
C G T C G A C G G C A G C T G C
SspI  (6041)
1 site
A A T A T T T T A T A A
ScaI  (5717)
1 site
A G T A C T T C A T G A
PvuI  (5607)
1 site
C G A T C G G C T A G C
AhdI  (5237)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4344)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BsmI  (3913)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
PfoI  (3768)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BssHII  (3390)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PflFI  (3111)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3111)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (2996)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BglII  (12)
1 site
A G A T C T T C T A G A
Bpu10I  (180)
1 site
C C T N A G C G G A